• Immunohistochemistry analysis of paraffin-embedded human placenta using CD55 Rabbit monoclonal antibody (STJ11101308) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CD55 Rabbit monoclonal antibody (STJ11101308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CD55 antibody (50-150) [S8MR] (STJ11101308)

SKU:
STJ11101308

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD55 (50-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD55
Gene ID: 1604
Uniprot ID: DAF_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CD55 (P08174).
Immunogen Sequence: EGRTSFPEDTVITYKCEESF VKIPGEKDSVICLKGSQWSD IEEFCNRSCEVPTRLNSASL KQPYITQNYFPVGTVVEYEC RPGYRREPSLSPKLTCLQNL K
Tissue Specificity Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Post Translational Modifications The Ser/Thr-rich domain is heavily O-glycosylated.
Function This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage. (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5. (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable). (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.
Protein Name Complement Decay-Accelerating Factor
Cd Antigen Cd55
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-6798695
Reactome: R-HSA-6807878
Reactome: R-HSA-977606
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cell Membrane
Lipid-Anchor
Gpi-Anchor
Isoform 3: Secreted
Isoform 4: Secreted
Isoform 5: Secreted
Isoform 6: Cell Membrane
Isoform 7: Cell Membrane
Alternative Antibody Names Anti-Complement Decay-Accelerating Factor antibody
Anti-Cd Antigen Cd55 antibody
Anti-CD55 antibody
Anti-CR antibody
Anti-DAF antibody

Information sourced from Uniprot.org

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