Signal Transduction | Research Area | St John's Laboratory

Immunohistochemistry analysis of E-cad. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:5000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.

Anti-E cadherin antibody [SM4912] (STJA0004912)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat/Monkey

$211.83 - $315.33

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Anti-E Cadherin antibody [SM5185] (STJA0005185)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$315.33

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Western blot analysis of various lysates using E-Cadherin Rabbit mAb (STJ11107256) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 30s.

Anti-E-Cadherin antibody (775-874 aa) [SRM5347] (STJ11107256)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $765.21

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Western blot A431 cells probed with anti-E-Cadherin antibody at multiple dilutions STJA0041146

Anti-E-Cadherin antibody (C-Term) (STJA0041146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$654.81

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Anti-E-Cadherin antibody [36B5] (STJ16101733)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$1,478.67

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Anti-E-Cadherin antibody [BS38] (STJ16101355)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Dog/Rat/Mouse/Pig/Sheep/Rabbit

$1,696.71

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Anti-E-Cadherin antibody [EP700Y] (STJ16100361)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$1,027.41 - $1,789.17

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Anti-E-CADHERIN antibody [MX020] (STJ16101857)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$2,457.09

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Anti-E-Cadherin antibody [SMM5478] (STJ11107419)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/ELISA

Reactivity :

Human

$174.57 - $765.21

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Anti-E-Cadherin antibody [SRM5109] (STJ11106906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $765.21

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Anti-E-Cadherin antibody IHC Human breast lobular carcinoma STJ180566

Anti-E-Cadherin antibody [ZR375] (STJ180566)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$202.17 - $745.89

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Anti-E-Cadherin/CD324 antibody {ABflo® 450} (STJ11108951)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

$264.27 - $744.51

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Anti-E-Cadherin/CD324 antibody {ABflo® 594} (STJ11108527)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

$275.31 - $665.85

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Anti-E-Cadherin/CD324 antibody {ABflo® 647} (STJ11108497)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

$275.31 - $665.85

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Anti-E-Cadherin/CD324 antibody {ABflo® 700} (STJ11108952)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

$297.39 - $856.29

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Immunofluorescence analysis of Hela cell. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 monoclonal antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-E2F1 antibody (100-170 aa) (STJ98642)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-E2F1 antibody (125-165) (STJ500811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$605.13

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Anti-E2F1 antibody (125-165) {Biotin} (STJ500815)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-E2F1 antibody (125-165) {FITC} (STJ500816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Immunofluorescence analysis of C6 cells using E2F1 Polyclonal Antibody (STJ23457) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-E2F1 antibody (200-301) (STJ23457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using E2F1 Rabbit polyclonal antibody (STJ119131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-E2F1 antibody (301-437) (STJ119131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-E2F1 antibody (338-437) [S9MR] (STJ11101709)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Anti-E2F1 antibody (380-430) (STJ500812)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$605.13

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Anti-E2F1 antibody (380-430) {Biotin} (STJ500813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-E2F1 antibody (380-430) {FITC} (STJ500814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Western blot with Anti-E2F1 Antibody (STJ500810) with peptide on at 1:500 dilution in buffer.

Anti-E2F1 antibody (58-93) (STJ500810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$605.13

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Anti-E2F1 antibody (58-93) {Biotin} (STJ500817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-E2F1 antibody (58-93) {FITC} (STJ500818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Western blot detection of E2F-1 in C6, Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.

Anti-E2F1 antibody [4G8-2C2-C12] (STJ99250)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Rat

$205.62 - $496.80

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E2F1 antibody western blot C6 Raw2647 HeLa STJA0032477

Anti-E2F1 antibody [4G8-2C2-C12] (STJA0032477)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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E2F1 antibody western blot MOLT4 STJA0034260

Anti-E2F1 antibody [R03-5G3] (STJA0034260)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of various lysates using E2F3 Rabbit pAb (STJ111428) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-E2F3 antibody (200-300) (STJ111428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using E2F4 antibody (STJ11103078) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-E2F4 antibody (250-350) [S8MR] (STJ11103078)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Western blot analysis of Jurkat cells using E2F-4 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-E2F4 antibody (61-110 aa) (STJ96687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-E2F4 antibody (89-198) (STJ11103181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$152.49 - $697.59

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E2F4 antibody immunohistochemistry paraffin-embedded endometrial tissue STJA0037740

Anti-E2F4 antibody [R08-5M7] (STJA0037740)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$425.73

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Anti-E2F4 antibody [R09-7E6] (STJA0034261)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$193.89 - $425.73

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Anti-E2F4 antibody [SQab30318] (STJ16103030)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$977.73

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Western blot analysis of HeLa cells using E2F-5 Polyclonal Antibody

Anti-E2F5 antibody (93-142 aa) (STJ92810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using EBAG9 antibody (STJ23462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-EBAG9 antibody (28-213) (STJ23462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Anti-EBAG9 antibody (STJA0008643)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of L929 cells using ECE1 Rabbit polyclonal antibody (STJ11104569) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ECE1 antibody (670-770) (STJ11104569)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from wild type (WT) and ECE1 knockout (KO) HeLa cells, using [KO Validated] ECE1 Rabbit pAb (STJ27605) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-ECE1 antibody (670-770) (STJ27605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-ECE1 antibody (STJ192263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-EDB/EDB-FN antibody [SAA1375] (STJA0019667)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA/IHC/WB

Reactivity :

Human

$269.79 - $1,820.91

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Anti-EDG1 antibody (STJA0037907)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$258.75 - $388.47

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EDG1 antibody western blot Jurkat cells STJA0036322

Anti-EDG1 antibody [R02-1C3] (STJA0036322)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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EDG2 antibody western blot A375 lysates STJA0036533

Anti-EDG2 antibody [R02-5G9] (STJA0036533)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

$184.23 - $388.47

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