• Anti-E2F1 antibody (338-437) [S9MR] (STJ11101709)
  • Immunoprecipitation analysis of 300 Mu g extracts of HT-29 cells using 3 Mu g E2F1 antibody (STJ11101709). Western blot was performed from the immunoprecipitate using E2F1 antibody (STJ11101709) at a dilution of 1:1000.
  • Western blot analysis of lysates from rat spleen, using E2F1 Rabbit monoclonal antibody (STJ11101709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.
  • Western blot analysis of various lysates using E2F1 Rabbit monoclonal antibody (STJ11101709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-E2F1 antibody (338-437) [S9MR] (STJ11101709)

SKU:
STJ11101709

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-E2F1 (338-437) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: E2F1
Gene ID: 1869
Uniprot ID: E2F1_HUMAN
Immunogen Region: 338-437
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 338-437 of human E2F1 (Q01094).
Immunogen Sequence: PPSSLTTDPSQSLLSLEQEP LLSRMGSLRAPVDEDRLSPL VAADSLLEHVREDFSGLLPE EFISLSPPHEALDYHFGLEE GEGIRDLFDCDFGDLTPLDF
Post Translational Modifications Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis. Phosphorylation at Ser-403 by GSK3B promotes interaction with USP11, leading to its deubiquitination and stabilization. Ubiquitinated via 'Lys-63'-linked ubiquitin, leading to its degradation. Deubiquitinated by USP11 follwong phosphorylation by GSK3B, promoting its stability. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. Methylation at Lys-185 by SETD7 promotes E2F1 ubiquitin-dependent proteasomal degradation.
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Directly activates transcription of PEG10. Positively regulates transcription of RRP1B.
Protein Name Transcription Factor E2f1
E2f-1
Pbr3
Retinoblastoma-Associated Protein 1
Rbap-1
Retinoblastoma-Binding Protein 3
Rbbp-3
Prb-Binding Protein E2f-1
Database Links Reactome: R-HSA-111448
Reactome: R-HSA-113501
Reactome: R-HSA-1362277
Reactome: R-HSA-1362300
Reactome: R-HSA-139915
Reactome: R-HSA-1912408
Reactome: R-HSA-2559580
Reactome: R-HSA-2559585
Reactome: R-HSA-6804116
Reactome: R-HSA-68911
Reactome: R-HSA-69202
Reactome: R-HSA-69205
Reactome: R-HSA-69231
Reactome: R-HSA-69656
Reactome: R-HSA-8953750
Reactome: R-HSA-9616222
Reactome: R-HSA-9661069
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor E2f1 antibody
Anti-E2f-1 antibody
Anti-Pbr3 antibody
Anti-Retinoblastoma-Associated Protein 1 antibody
Anti-Rbap-1 antibody
Anti-Retinoblastoma-Binding Protein 3 antibody
Anti-Rbbp-3 antibody
Anti-Prb-Binding Protein E2f-1 antibody
Anti-E2F1 antibody
Anti-RBBP3 antibody

Information sourced from Uniprot.org

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