Signal Transduction | Research Area | St John's Laboratory

Western blot analysis using Akt2 monoclonal antibody against A431 (1) , Jurkat (2) , HEK293 (3) , A549 (4) , MCF-7 (5) and PC-12 (6) cell lysate.

Anti-AKT2 antibody [1B6] (STJ97821)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Monkey

£149.00 - £360.00

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Anti-AKT2 antibody [SRM14707] (STJ11109437)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various lysates using AKT3 Rabbit polyclonal antibody (STJ114775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-AKT3 antibody (90-465) (STJ114775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using AKT3 antibody (STJ11104934) at 1:60000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AKT3 antibody (93-154 aa) [S4934RM] (STJ11104934)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of 1) Hela, 2) 3T3, 3) Mouse Brain, 4) Rat Brain tissue, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-AKT3 antibody (STJ197445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using Akt3 monoclonal antibody against truncated Akt3 recombinant protein (1) and human ovary carcinoma tissue lysate (2).

Anti-AKT3 antibody [3B12F3F10/2D2B11B11/2D2B11H5] (STJ97822)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-AKT3 nanobody [SAA1232] (STJN000317)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

IF

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of lysates from Mouse lung, using Alcohol Dehydrogenase Rabbit monoclonal antibody (STJ11103058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Alcohol Dehydrogenase antibody (1-100) [S8MR] (STJ11103058)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of mouse lung, mouse liver cells using ALDH1A1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ALDH1A1 antibody (21-70 aa) (STJ96522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-ALDH1A1 antibody (250-350 aa) [SRM5086] (STJ11106867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of various lysates, using ALDH1A1 rabbit monoclonal antibody (STJ11101231) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ALDH1A1 antibody (250-350) [S1MR] (STJ11101231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using ALDH1A1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-ALDH1A1 antibody (328-343 aa) [R09-3B9] (STJA0010773)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

£264.50

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Immunofluorescence analysis of HepG2 cells using ALDH1A1 Rabbit polyclonal antibody (STJ22577) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ALDH1A1 antibody (329-345) (STJ22577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Formalin-fixed, paraffin-embedded human solitary fibrous tumor stained with anti-ALDH1A1 monoclonal antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic/nuclear staining of tumor cells

Anti-ALDH1A1 antibody (Full length) [ZM71] (STJ180485)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £564.50

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Anti-ALDH1A1 antibody (N-Term) [M558] (STJA0005254)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-ALDH1A1 antibody (N-Term) [M562] (STJA0005255)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£391.50

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Western blot analysis using ALDH1A1 monoclonal antibody against Raji (1) , Jurkat (2) , THP-1 (3) and K562 (4) cell lysate.

Anti-ALDH1A1 antibody [5A11] (STJ97824)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-ALDH1A1 antibody [PT0629R] (STJA0039297)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-ALDH1A1 antibody [SRM14526] (STJ11109256)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Rat

£0.00

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Western blot analysis of various lysates using ALDH1A2 Rabbit mAb (STJ11102426) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ALDH1A2 antibody (1-100) [S6MR] (STJ11102426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of NIH-3T3 cells using ALDH1A2 antibody (STJ29639) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ALDH1A2 antibody (1-180) (STJ29639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from K562 cells, using ALDH1A2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ALDH1A2 antibody (412-461 aa) (STJ91553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from mouse pancreas, using ALDH1A3 Rabbit polyclonal antibody (STJ117424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ALDH1A3 antibody (1-140) (STJ117424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-ALDH1A3 antibody (290-370 aa) (STJ190531)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from wild type (WT) and ALDH3A2 knockout (KO) HeLa cells, using [KO Validated] ALDH3A2 Rabbit polyclonal antibody (STJ11100775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ALDH3A2 antibody (250-320) (STJ11100775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of L929 cells using ALDH3A2 Rabbit polyclonal antibody (STJ118702) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALDH3A2 antibody (250-320) (STJ118702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis using ALDH3A2 monoclonal antibody against A549, U251, HepG2 cell lysate.

Anti-ALDH3A2 antibody (STJ98463)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Rabbit

£149.00 - £360.00

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Western blot detection of Aldehyde dehydrogenase 10 in A549, U251 and HepG2 cell lysates using Aldehyde dehydrogenase 10 mouse mAb (1:1000 diluted).Predicted band size:55KDa.Observed band size:55KDa.

Anti-ALDH3A2 antibody [3F7-H9-E12] (STJ99136)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£149.00 - £360.00

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Anti-ALDH8A1 antibody (STJA0004762)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Anti-ALDH8A1 antibody (STJA0004763)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Anti-ALDH8A1 antibody (STJA0008661)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates, using ALK antibody (STJ11104956) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ALK antibody (1334-1520 aa) [S4956RM] (STJ11104956)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of HeLa cells, using ALK Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ALK antibody (1570-1619 aa) (STJ91560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of Neuro-2a cells using ALK Rabbit polyclonal antibody (STJ116404) at dilution of 1:200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALK antibody (1603-1620) (STJ116404)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-ALK antibody (STJ11108288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£0.00

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Anti-ALK antibody [ALK-1] (STJ16100308)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,197.50 - £1,870.50

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Anti-ALK antibody [PT0548R] (STJA0039446)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human

£285.00 - £540.00

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Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALKAL2 antibody (64-114 aa) (STJ195351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Alpha Fodrin antibody [SRM13379] (STJ11108109)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-alpha smooth muscle Actin antibody [SM0388] (STJA0010388)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-alpha smooth muscle Actin antibody [SM0389] (STJA0010389)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Immunohistochemistry analysis of paraffin-embedded human kidney using Alpha-SMA at dilution of 1:500.

Anti-alpha smooth muscle Actin antibody [SM0514] (STJA0010514)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£233.50

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Anti-Alpha-Actinin antibody [SRM13488] (STJ11108218)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Alpha-Smooth Muscle Actin (ACTA2) antibody [SRM13045] (STJ11107775)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various lysates using Alpha-Tubulin Rabbit pAb (STJ11103685) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-Tubulin antibody (1-180) (STJ11103685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£156.50 - £290.50

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (STJ11103689) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) (STJ11103689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (STJ11103690) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) (STJ11103690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£201.50 - £379.50

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit mAb (STJ11103694) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) [S4MR] (STJ11103694)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £638.50

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