Signal Transduction | Research Area | St John's Laboratory

Anti-HIF1a antibody at 2500 dilution; HT1080 cells in the presence of CoCl2; lysate at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-HIF1A antibody (470 to 590aa) (STJ140124)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Western blot analysis of lysates from HepG2 cells, using HIF1 Alpha Rabbit polyclonal antibody (STJ29997) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HIF1A antibody (600-826) (STJ29997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from HeLa cells, using HIF1 Alpha Rabbit pAb (STJ113863) at 1:1000 dilution. HeLa cells were treated by Cobalt chloride (0. 1 mM) at 37 °C for 4 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-HIF1A antibody (670-826) (STJ113863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HIF1A antibody (780aa C-Term) (STJ140040)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Western blot analysis using HIF-1 Alpha monoclonal antibody against Cos7 (1) , HeLa (2) , Jurkat (3) , RAJI (4) and NIH/3T3 (5) cell lysate.

Anti-HIF1A antibody [1A3] (STJ98135)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

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Western blot analysis of 1) HepG2 Cell, 2) HepG2 Treated with CoCl2 cell lysate using HIF-1α mouse Monoclonal monoclonal antibody diluted at 1:2,000.

Anti-HIF1A antibody [Mix] (STJ196669)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-HIF1A antibody [SAA0814] (STJA0019626)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-HIF1A nanobody [SAA1239] (STJN000280)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-HINT2 antibody (STJA0004739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Anti-HINT2 antibody (STJA0004740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Anti-HINT2 antibody (STJA0004741)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human

£140.50 - £415.50

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Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (STJ29383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Histone H2A antibody (1-143) (STJ29383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Western blot analysis of various lysates using Histone H2AX Rabbit pAb (STJ113097) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Histone H2A antibody (50-143) (STJ113097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Anti-Histone H2AX antibody (1-143 aa) (STJ11106040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Anti-Histone H2B antibody [SAA1806] (STJA0020234)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and Histone H3. 3 rabbit monoclonal antibody (STJ11102163). The ChIP sequencing results indicate the enrichment pattern of Histone H3. 3 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Histone H3 antibody (57-136) [S3MR] (STJ11102163)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Anti-Histone H3 antibody [SM0412] (STJA0010412)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Histone H3.2 antibody (81-131 aa) (STJ194163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using [KO Validated] Histone H4 Rabbit pAb (STJ11106815) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Histone H4 antibody (1-100 aa) (STJ11106815)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using Histone H4 Rabbit polyclonal antibody (STJ119273) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Histone H4 antibody (1-100) (STJ119273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Western blot analysis of various lysates, using Histone H4 Rabbit polyclonal antibody (STJ29382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Histone H4 antibody (1-100) (STJ29382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Immunohistochemistry analysis of Histone H4 in paraffin-embedded mouse kidney using Histone H4 Rabbit monoclonal antibody (STJ11103155) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Histone H4 antibody (24-103) [S5MR] (STJ11103155)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Immunocytochemistry analysis of Histone H4 (green) in Hela using Histone H4 antibody, and DAPI (blue).

Anti-Histone H4 antibody (51-70 aa) [R05-8H9] (STJA0011428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/ChIP

Reactivity :

Human

£246.50

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Immunofluorescence analysis of human-liver tissue. 1, Histone H4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Histone H4 antibody (6-55 aa) (STJ93539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of various lysates using HMOX2 Rabbit polyclonal antibody (STJ24054) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HMOX2 antibody (1-270) (STJ24054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HMOX2 antibody (STJ192960)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and hnRNP A1 knockout (KO) 293T cells, using [KO Validated] hnRNP A1 Rabbit polyclonal antibody (STJ11100067) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HNRNPA1 antibody (1-140) (STJ11100067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of A549 cells using hnRNP A1 antibody (STJ29627).

Anti-HNRNPA1 antibody (1-372) (STJ29627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g hnRNP A1 antibody (STJ11101361). Western blot was performed from the immunoprecipitate using hnRNP A1 antibody (STJ11101361) at a dilution of 1:1000.

Anti-HNRNPA1 antibody (250-350) [S1MR] (STJ11101361)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various cells using hnRNP A1 Polyclonal Antibody diluted at 1：2000

Anti-HNRNPA1 antibody (51-100 aa) (STJ93557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293T cells using hnRNP C1/2 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-HNRNPC antibody (231-280 aa) (STJ93559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using hnRNP C1/C2 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-HNRNPC antibody (241-290 aa) (STJ93560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using hnRNP C Rabbit mAb (STJ11102402) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-HNRNPC antibody (80-170) [S2MR] (STJ11102402)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunofluorescence analysis of U-2 OS cells using hnRNP C Rabbit polyclonal antibody (STJ11100595) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HNRNPC antibody (86-190) (STJ11100595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using HNRNPF antibody (STJ27456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HNRNPF antibody (1-280) (STJ27456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of various cells using hnRNP F Polyclonal Antibody

Anti-HNRNPF antibody (11-60 aa) (STJ93564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates, using HNRNPF Rabbit polyclonal antibody (STJ11104962) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HNRNPF antibody (315-415 aa) (STJ11104962)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-HNRNPH1 antibody (191-449) (STJ27720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of MCF7 cells, using hnRNP M Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-HNRNPM antibody (11-60 aa) (STJ93570)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using hnRNPM antibody (STJ29017) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HNRNPM antibody (491-730) (STJ29017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HPN antibody (350-430 aa) (STJ192347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-HPN antibody (45-170 aa) (STJ11107442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-HPN/Hepsin antibody [SAA1938] (STJA0020366)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of various lysates using HRAS Rabbit pAb (STJ110210) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 20s.

Anti-HRas antibody (1-100) (STJ110210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using HRAS Rabbit polyclonal antibody (STJ114104) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HRAS antibody (1-189) (STJ114104)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-HRAS antibody (100-150) (STJ502032)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Chicken/Human/Mouse

£427.50

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Anti-HRAS antibody (100-150) {Biotin} (STJ502030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Chicken/Human/Mouse

£528.50

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Anti-HRAS antibody (100-150) {FITC} (STJ502031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Chicken/Human/Mouse

£528.50

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