Programmed Cell Death | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using Ubiquitin antibody (STJ11101766) at1:1000 dilution. HeLa, NIH/3T3 and C6 cell were treated by MG132 (50 Mu M) at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-UBB antibody (1-228) [S6MR] (STJ11101766)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Immunofluorescence analysis of HeLa cells using Ubiquitin Rabbit polyclonal antibody (STJ11100145) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-UBB antibody (1-229) (STJ11100145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Western blot analysis of lysates from C6 cells using Ubiquitin Rabbit polyclonal antibody (STJ26009) at 1:900 dilution. C6 cells were treated by MG132 (50 Mu M) at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-UBB antibody (1-229) (STJ26009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of Ubiquitin in paraffin-embedded human placenta using Ubiquitin Rabbit polyclonal antibody (STJ26010) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-UBB antibody (1-229) (STJ26010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Immunofluorescence analysis of HepG2 cells using UBC Rabbit polyclonal antibody (STJ29897) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-UBC antibody (1-100) (STJ29897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UBC antibody (160-210 aa) (STJ194141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH-3T3 cells using UBE2L3 Polyclonal Antibody (STJ26020) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-UBE2L3 antibody (73-212) (STJ26020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UBE2L3 antibody (STJ191597)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-UNC5A antibody (130-210 aa) (STJ191048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-UNC5A antibody (230-300 aa) (STJ11106511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using UNC5B Rabbit polyclonal antibody (STJ117177) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-UNC5B antibody (290-377) (STJ117177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-UNC5B antibody (350-430 aa) (STJ191049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Vimentin antibody (STJ26092) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-VIM antibody (1-200) (STJ26092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit polyclonal antibody (STJ113868) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-VIM antibody (1-466) (STJ113868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of U-2 OS cells using Vimentin Rabbit polyclonal antibody (STJ11104460) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-VIM antibody (10-101) (STJ11104460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of Vimentin in paraffin-embedded rat spleen using Vimentin Rabbit polyclonal antibody (STJ113129) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-VIM antibody (300-400) (STJ113129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-VIM antibody (367-466) [S7MR] (STJ11101727)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-VIM antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-VIM antibody (390aa C-Term) (STJ140133)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Human appendix tissue was stained with Anti-Vimentin (ABT019) Antibody

Anti-VIM antibody (400-466 aa) [ABT019] (STJ197249)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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U2OS whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Vimentin (ABT281) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: U2OSPredicted band size: 54kDaObserved band size: 54kDa

Anti-VIM antibody (400-466 aa) [ABT281] (STJ197198)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunocytochemistry analysis of Vimentin (green) in Hela using Vimentin antibody, and DAPI (blue).

Anti-VIM antibody (448-465 aa) [R02-8C4] (STJA0013017)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat/Hamster

£264.50

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Immunofluorescence analysis of Hela cell. 1, Vimentin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-VIM antibody (56-105 aa) (STJ96245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain tissue, diluted at 1:2000.

Anti-VIM antibody [1A7] (STJ97060)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of Vimentin in Molt-4, K562, COS7, Jurkat, Hela and Vimentin negative cell (Daudi, Ramos, Raji) lysates using Vimentin mouse mAb (1:1000 diluted).Predicted band size:57KDa.Observed band size:57KDa.

Anti-VIM antibody [4F8-A5-H5] (STJ99270)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human

£144.00 - £348.00

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Formalin-fixed, paraffin-embedded human gastrointestinal stromal tumor stained with anti-vimentin antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells

Anti-Vimentin antibody (2-466aa) [ZR381] (STJ180548)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£161.50 - £496.50

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Immunostaining of tumors and precursor PanIN lesions from 4 week old LSL-KrasG12D; Cdkn2alox/lox; p48Cre (KIC) mice that were treated with 2G8 and/or gemcitabine, Gem. (Anti-Vimentin, cat. STJA0003823, green; Anti-E-cadherin, red). Photo courtesy of Rolf Brekken, University of Texas Southwestern. CC-BY-4. 0

Anti-Vimentin antibody (STJA0003823)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Vole

£403.50

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.

Anti-Vimentin antibody [2D1] (STJA0003824)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/D.melanogaster/Feline/Finch/Fish/Goat/Guinea Pig/Hamster/Horse/Lizard/Non-Human Primates/Rabbit/Sheep/Vole/Xenopus/Zebrafish

£454.50

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Anti-Vimentin antibody [65E] (STJ16100819)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/WB

Reactivity :

Human

£606.50

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Anti-Vimentin antibody [EBS-O-184] (STJ16102604)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human

£307.50 - £1,449.50

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Anti-Vimentin antibody [LN-6] (STJ16101027)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Cat/Cow/Human/Mouse/Pig/Rabbit/Rat/Sheep

£307.50 - £1,449.50

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Anti-Vimentin antibody [LN6] (STJA0003872)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human

£454.50

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Anti-Vimentin antibody [SM0430] (STJA0010430)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Anti-Vimentin antibody [SM5190] (STJA0005190)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Anti-Vimentin antibody [SRL33] (STJ16101801)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£748.50

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Anti-Vimentin antibody [V9] (STJ16100234)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IHC-F/WB/FC

Reactivity :

Human

£273.50

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Anti-Vimentin antibody [V9] (STJ16101600)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£567.50

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Anti-Vimentin antibody [V9] (STJ16101831)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£842.50

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Anti-Vimentin antibody [VI-RE/1] (STJ16100215)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/FC/WB/ICC/ELISA

Reactivity :

Human

£485.50

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Immunofluorescence analysis of HeLa cells using XIAP Rabbit polyclonal antibody (STJ115242). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-XIAP antibody (1-200) (STJ115242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts from normal (control) and XIAP knockout (KO) HeLa cells, using XIAP antibody (STJ11104084) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-XIAP antibody (1-200) [S4084RM] (STJ11104084)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of extracts of various cell lines, using XIAP antibody (STJ28949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-XIAP antibody (198-497) (STJ28949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using XIAP Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Anti-XIAP antibody (53-102 aa) (STJ96278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and XIAP knockout (KO) 293T cells, using [KO Validated] XIAP Rabbit monoclonal antibody (STJ11104809) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-XIAP antibody [S4809RM] (STJ11104809)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of extracts of various cell lines, using 14-3-3 alpha/beta rabbit monoclonal antibody (STJ11102440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-YWHAB antibody (147-246) [S0MR] (STJ11102440)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of L929 cells using 14-3-3 alpha/beta Rabbit polyclonal antibody (STJ26141) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-YWHAB antibody (150-246) (STJ26141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using 14-3-3 Beta Polyclonal Antibody

Anti-YWHAB antibody (41-90 aa) (STJ91367)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using 14-3-3 epsilon Rabbit monoclonal antibody (STJ11102200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-YWHAE antibody (1-100) [S0MR] (STJ11102200)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using 14-3-3 Epsilon Polyclonal Antibody diluted at 1：1000

Anti-YWHAE antibody (206-255 aa) (STJ91370)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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