Metabolism | Research Area | St John's Laboratory

Anti-SMPD1 antibody (STJ192956)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-SMPD1 antibody [SRM14694] (STJ11109424)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

$163.53 - $719.67

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Western blot analysis of various lysates using SMPD2 Rabbit polyclonal antibody (STJ25629) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SMPD2 antibody (150-250) (STJ25629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using SMPD3 antibody (STJ112235) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SMPD3 antibody (416-655) (STJ112235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-SMPD3 antibody (C-Term) (STJA0005587)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$507.15

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Anti-SMPD3/NSMase2 antibody [SRM14635] (STJ11109365)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunofluorescence analysis of L929 cells using SMPD4 Rabbit polyclonal antibody (STJ117668) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SMPD4 antibody (600-800) (STJ117668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of various lysates using SMS Rabbit pAb (STJ111588) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-SMS antibody (1-366) (STJ111588)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of various lysates using (STJ11105753) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-SMS antibody (50-150) [S5753RM] (STJ11105753)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from CACO2 cells, primary antibody was diluted at 1:1000, 4°C overnight STJ195132

Anti-SMS antibody (81-131 aa) (STJ195132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-SNAP-25 (BoTox-A Cleaved) antibody [4F3-2C1] (STJA0032123)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/IP/CBA

Reactivity :

Human

$507.15

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Immunofluorescence analysis of mouse brain using SNAP25 Rabbit polyclonal antibody (STJ25637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SNAP25 antibody (1-206) (STJ25637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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ICC IF of SNAP25 in U87MG cells stained with STJA0041539 antibody Alexa Fluor 488 secondary green and DAPI blue

Anti-SNAP25 antibody (1-206aa) [4E11] (STJA0041539)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

$302.91 - $458.85

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Western blot analysis of extracts of various cell lines, using SNAP25 Rabbit monoclonal antibody (STJ11101797) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SNAP25 antibody (107-206) [S7MR] (STJ11101797)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$163.53 - $719.67

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SNAP25 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-SNAP25 antibody (151-200 aa) (STJ95717)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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SNAP25 antibody immunocytochemistry HeLa cells STJA0013092

Anti-SNAP25 antibody (193-206 aa) [R07-1D7] (STJA0013092)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-SNAP25 antibody [SAA1653] (STJA0020081)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$273.93 - $1,615.29

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ICC IF of SORD in Hep3B cells with Alexa Fluor 488 and DAPI staining

Anti-SORD antibody (1-357aa) [AT10F4] (STJA0041675)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

$302.91 - $458.85

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Western blot analysis of extracts of HT-29 cells, using Sorbitol Dehydrogenase antibody (STJ11104612) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SORD antibody (100-200) [S4612RM] (STJ11104612)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunofluorescence analysis of NIH/3T3 cells using Sorbitol Dehydrogenase Rabbit polyclonal antibody (STJ25655) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SORD antibody (145-300) (STJ25655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SORD antibody (STJ194029)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of NIH/3T3 cells using SP1 Rabbit polyclonal antibody (STJ116866) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SP1 antibody (420-620) (STJ116866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ Hep G2 cells with 1 Mu g SP1 rabbit monoclonal antibody (STJ11101745) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of SP1 in representative gene loci (TINF2) , as shown in figure.

Anti-SP1 antibody (500-600) [S5MR] (STJ11101745)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq/CUT&Tag

Reactivity :

Human/Mouse/Rat

$174.57 - $765.21

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Immunohistochemistry analysis of SP1 in paraffin-embedded Human small intestine tissue using SP1 Rabbit pAb (STJ11107080) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-SP1 antibody (581-680 aa) (STJ11107080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various cells using Sp1 Polyclonal Antibody

Anti-SP1 antibody (706-755 aa) (STJ95737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-SP1 antibody [3C4C3] (STJ16103626)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/FC

Reactivity :

Human

$1,030.17

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Anti-SP1 antibody [PT0149R] (STJA0039722)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human

$207.00 - $745.20

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SP1 antibody immunocytochemistry HeLa cells STJA0034879

Anti-SP1 antibody [R01-9G6] (STJA0034879)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse

$184.23 - $388.47

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SP1 antibody immunohistochemistry human colon cancer STJA0034880

Anti-SP1 antibody [R06-9I9] (STJA0034880)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Immunofluorescence analysis of paraffin-embedded human testis using SPAM1 Rabbit polyclonal antibody (STJ25670) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SPAM1 antibody (100-300) (STJ25670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-SPAM1 antibody (STJA0009036)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-SPAM1 antibody [PT0768R] (STJA0039329)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human

$207.00 - $745.20

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Anti-SPAM1 antibody [SRM14501] (STJ11109231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Rat

$163.53 - $719.67

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Spermine Synthase antibody western blot K562 HeLa STJA0034881

Anti-Spermine Synthase antibody [R05-5J5] (STJA0034881)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$184.23 - $388.47

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Western blot recombinant human SK1 protein probed with anti-SK1 Central Region antibody STJA0041398

Anti-Sphingosine Kinase 1 antibody (Central Region) (STJA0041398)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

$748.65

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Western blot anti-SK2 antibody detection in recombinant SK2 and HeLa cell lysates STJA0041406

Anti-Sphingosine Kinase 2 antibody (N-Term) (STJA0041406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/IP

Reactivity :

Human/Mouse/Rat

$748.65

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Western blot human recombinant SK2 detection using antibody at various dilutions STJA0041386

Anti-Sphingosine Kinase 2 antibody (N-Term) [M532] (STJA0041386)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$748.65

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Western blot analysis of extracts of various cell lines, using SPHK1 antibody (STJ11104702) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SPHK1 antibody (1-160) [S4702RM] (STJ11104702)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SPHK1 antibody (160-240 aa) (STJ190139)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of SPHK1 in paraffin-embedded Mouse kidney tissue using SPHK1 Rabbit pAb (STJ11107091) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-SPHK1 antibody (201-300 aa) (STJ11107091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using SPHK1 antibody (STJ25673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-SPHK1 antibody (50-384) (STJ25673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Anti-SPHK1 antibody (Central region) (STJA0005594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$507.15

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SPHK1 antibody western blot Raji STJA0036490

Anti-SPHK1 antibody [R07-6N6] (STJA0036490)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of extracts of mouse brain, using SPHK2 antibody (STJ11100900). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-SPHK2 antibody (1-165) (STJ11100900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of extracts of Raji cells, using SPHK2 antibody (STJ28831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPHK2 antibody (1-165) (STJ28831)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of MCF7 cells using SPHK2 Rabbit pAb (STJ11107102) at a dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SPHK2 antibody (480-654 aa) (STJ11107102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of 22RV1 cells using SphK2 Polyclonal Antibody diluted at 1：500

Anti-SPHK2 antibody (580-629 aa) (STJ95748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-SPHK2 antibody (N-Term) (STJA0005599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

$507.15

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