Anti-Sphingosine Kinase 1 antibody (Central Region) (STJA0041398)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSK1 synthetic peptide (coupled to carrier protein) corresponding to an unphosphorylated sequence of amino acids surrounding serine 225 in the central region of human SK1. This sequence has 4 amino acid differences from mouse and 5 from rat SK1, and i
General Information
| Short Description | Rabbit polyclonal anti-Sphingosine Kinase 1 (Central Region) for use in ELISA and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20ยฐC is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20ยฐC. |
Target Information
| Gene Symbol | SPHK1 |
| Gene ID | 8877 |
| Uniprot ID | SPHK1_HUMAN |
| Immunogen | SK1 synthetic peptide (coupled to carrier protein) corresponding to an unphosphorylated sequence of amino acids surrounding serine 225 in the central region of human SK1. This sequence has 4 amino acid differences from mouse and 5 from rat SK1, and i |
| Immunogen Region | Central Region |
| Immunogen Sequence | Human |
| Specificity | The antibody detects a 47 kDa* full-length recombinant human SK1 protein. In mouse, the antibody detects SK1 in isolated cardiac myocytes, but not in SK1-null mice (Tao, R. et al. (2007) Cardiovas. Res. 74:56.). |
Additional Info
| Tissue Specificity | Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level). |
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion. In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA. Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. |
| Protein Name | Sphingosine Kinase 1Sk 1Spk 1Acetyltransferase Sphk1 |
| Database Links | Reactome: R-HSA-1660661Reactome: R-HSA-390471Reactome: R-HSA-5218921Reactome: R-HSA-9009391Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmNucleusCell MembraneEndosome MembranePeripheral Membrane ProteinMembraneClathrin-Coated PitSynapseTranslocated From The Cytoplasm To The Plasma Membrane In A Cib1-Dependent MannerBinds To Membranes Containing Negatively Charged Lipids But Not Neutral LipidsRecruited To Endocytic Membranes By Sphingosine Where Promotes Membrane Fusion |
| Alternative Antibody Names | Anti-Sphingosine Kinase 1 antibodyAnti-Sk 1 antibodyAnti-Spk 1 antibodyAnti-Acetyltransferase Sphk1 antibodyAnti-SPHK1 antibodyAnti-SK1 antibodyAnti-SPHK antibodyAnti-SPK antibody |
Information sourced from Uniprot.org