• Immunofluorescence analysis of paraffin-embedded human testis using SPAM1 Rabbit polyclonal antibody (STJ25670) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat testis using SPAM1 Rabbit polyclonal antibody (STJ25670) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SPAM1 in paraffin-embedded rat testis using SPAM1 Rabbit polyclonal antibody (STJ25670) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SPAM1 Rabbit polyclonal antibody (STJ25670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SPAM1 antibody (100-300) (STJ25670)

SKU:
STJ25670

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPAM1 (100-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPAM1
Gene ID: 6677
Uniprot ID: HYALP_HUMAN
Immunogen Region: 100-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2).
Immunogen Sequence: PYIDSITGVTVNGGIPQKIS LQDHLDKAKKDITFYMPVDN LGMAVIDWEEWRPTWARNWK PKDVYKNRSIELVQQQNVQL SLTEATEKAKQEFEKAGKDF LVETIKLGKLLRPNHLWGYY LFPDCYNHHYKKPGYNGSCF NVEIKRNDDLSWLWNESTAL YPSIYLNTQQSPVAATLYVR NRVREAIRVSKIPDAKSPLP V
Tissue Specificity Testis.
Post Translational Modifications N-glycosylated.
Function Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Protein Name Hyaluronidase Ph-20
Hyal-Ph20
Hyaluronoglucosaminidase Ph-20
Sperm Adhesion Molecule 1
Sperm Surface Protein Ph-20
Database Links Reactome: R-HSA-2534343
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Hyaluronidase Ph-20 antibody
Anti-Hyal-Ph20 antibody
Anti-Hyaluronoglucosaminidase Ph-20 antibody
Anti-Sperm Adhesion Molecule 1 antibody
Anti-Sperm Surface Protein Ph-20 antibody
Anti-SPAM1 antibody
Anti-HYAL3 antibody
Anti-PH20 antibody

Information sourced from Uniprot.org

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