Metabolism | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using ALDH7A1 antibody (STJ11104095) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ALDH7A1 antibody (1-100) [S4095RM] (STJ11104095)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunocytochemistry analysis of ALDH7A1 (green) in Hela using ALDH7A1 antibody, and DAPI (blue)

Anti-ALDH7A1 antibody (16-29 aa) [R01-9D6] (STJA0010929)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

£246.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALDH7A1 antibody (229-279 aa) (STJ195163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using ALDH7A1 Rabbit pAb (STJ111351) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Negative control (NC) : K-562. <br/>Exposure time: 75s.

Anti-ALDH7A1 antibody (430-539) (STJ111351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from wild type (WT) and ALDH9A1 knockout (KO) 293T cells, using [KO Validated] ALDH9A1 Rabbit pAb (STJ110185) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-ALDH9A1 antibody (1-180) (STJ110185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ALDH9A1 antibody (256-450 aa) [SRM5257] (STJ11107159)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Anti-ALDH9A1 antibody (STJA0008251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of A549 cells using ALDOA antibody (STJ22584). Blue: DAPI for nuclear staining.

Anti-ALDOA antibody (1-348) (STJ22584)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using Aldolase A Polyclonal Antibody diluted at 1：1000

Anti-ALDOA antibody (1-50 aa) (STJ91556)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ALDOA antibody [SRM13029] (STJ11107759)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of extracts of various cell lines, using ALDOB antibody (STJ22585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ALDOB antibody (1-200) (STJ22585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using Aldolase B Polyclonal Antibody

Anti-ALDOB antibody (111-160 aa) (STJ91557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 cells using ALDOC Rabbit polyclonal antibody (STJ113223) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALDOC antibody (1-170) (STJ113223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of mouse liver, mouse spinal cord cells using Aldolase C Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ALDOC antibody (21-70 aa) (STJ96549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Alkaline Phosphatase (intestinal) antibody [V17.1] (STJ16100829)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC

Reactivity :

Cow/Human

£287.50 - £1,324.50

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Western blot analysis of A-431, using ALOX12 antibody (STJ116903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-ALOX12 antibody (483-663) (STJ116903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ALOX12 antibody (N-Term) (STJA0010318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-ALOX12 antibody [SAA1590] (STJA0020018)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of various lysates using ALOX12B Rabbit polyclonal antibody (STJ118650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALOX12B antibody (1-250) (STJ118650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of PC-12 cells using ALOX15 Rabbit polyclonal antibody (STJ28944) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALOX15 antibody (1-230) (STJ28944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALOX15 antibody (156-206 aa) (STJ195947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-ALOX15 antibody [SRM14578] (STJ11109308)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£0.00

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Immunofluorescence analysis of U2OS cells using ALOX15B Rabbit polyclonal antibody (STJ28945) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALOX15B antibody (1-351) (STJ28945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALOX15B antibody (576-626 aa) (STJ195383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of C6 cells using ALOX5 antibody (STJ22592) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ALOX5 antibody (1-200) (STJ22592)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Confocal imaging of U-2 OS cells using ALOX5 Rabbit monoclonal antibody (STJ11101834, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-ALOX5 antibody (100-200) [S4MR] (STJ11101834)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Arachidonate 5 Lipoxygenase Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ALOX5 antibody (246-295 aa) (STJ91392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ALOX5 antibody [SRM14754] (STJ11109484)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-ALOX5AP antibody (STJA0009801)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using ALOXE3 Rabbit pAb (STJ110544) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-ALOXE3 antibody (1-280) (STJ110544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ALOXE3 antibody (467-517) (STJA0006307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of 293F-ALPI, using ALPI rabbit polyclonal antibody (STJ27982) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ALPI antibody (20-527) (STJ27982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-ALPI antibody (STJ192955)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of mouse kidney using AMACR Rabbit polyclonal antibody (STJ22599) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AMACR antibody (1-138) (STJ22599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot with Anti-AMACR Antibody (STJ500070) of HEK293 cell at 1:500 dilution in buffer.

Anti-AMACR antibody (260-310) (STJ500070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/DB/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse

£427.50

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Anti-AMACR antibody (260-310) {Biotin} (STJ500071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/DB/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse

£528.50

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Anti-AMACR antibody (260-310) {FITC} (STJ500072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/DB/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse

£528.50

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Anti-AMACR antibody (271-320) (STJA0010158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Whole cell lysates of LnCap were separated by 8% SDS-PAGE, and the membrane was blotted with anti-AMACR antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 42 kDa

Anti-AMACR antibody (300-382 aa) [ABT-AMACR] (STJ196992)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£180.00 - £261.00

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Anti-AMACR antibody (300-382) [ABT-AMACR] (STJA0039084)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF/ELISA

Reactivity :

Human

£49.00 - £261.00

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Immunohistochemistry of rat kidney with Anti-AMACR primary antibody (STJ500069) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 20X.

Anti-AMACR antibody (50-100) (STJ500069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/DB/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£427.50

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Anti-AMACR antibody (50-100) {Biotin} (STJ500073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/DB/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£528.50

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Anti-AMACR antibody (50-100) {FITC} (STJ500074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/DB/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£528.50

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Western blot detection of AMACR (C-terminus) in Rat Liver lysates using AMACR (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 42KDa.Observed band size: 42KDa.

Anti-AMACR antibody (C-Term) [2F3-C11-B12] (STJ99109)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Rat

£149.00 - £360.00

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Human prostate carcinoma stained anti-AMACR antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and luminal circumferential staining of carcinoma glands.

Anti-AMACR antibody [13H4] (STJ180006)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£202.50 - £856.50

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Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, AMACR monoclonal antibody (4A12) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-AMACR antibody [4A12] (STJ96949)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of AMACR at dilution of 1:1000

Anti-AMACR antibody [SM0426] (STJA0010426)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Anti-AMACR antibody [SRM13078] (STJ11107808)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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