Metabolism | Research Area | St John's Laboratory

Anti-AKT antibody (STJ13100006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

$447.53

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Western blot analysis of extracts of various cell lines, using AKT1 antibody (STJ27474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AKT1 antibody (100-200) (STJ27474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-Akt1 antibody (251-351 aa) [SRM] (STJ11106868)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of extracts of MCF7 cells, using AKT1 antibody (STJ112889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-AKT1 antibody (350-480) (STJ112889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.

Anti-AKT1 antibody (370-477 aa) [10D6] (STJ97724)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat/D.melanogaster

$64.80 - $340.20

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Immunofluorescence analysis of PC-12 cells using [KO Validated] AKT1 Rabbit polyclonal antibody (STJ11104319) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AKT1 antibody (380-480) (STJ11104319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Confocal imaging of MCF7 cells using [KO Validated] AKT1 Rabbit monoclonal antibody (STJ11104954, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-AKT1 antibody (380-480) [S4954RM] (STJ11104954)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunoprecipitation analysis of 300 Mu g extracts from MCF7 cells using 3 Mu g AKT1 Rabbit monoclonal antibody (STJ11101453). Western blot was performed from the immunoprecipitate using AKT1 Rabbit monoclonal antibody (STJ11101453) at a dilution of 1:1000.

Anti-AKT1 antibody (381-480) [S3MR] (STJ11101453)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of extracts from wild type (WT) and AKT1 knockout (KO) HeLa cells, using AKT1 antibody (STJ11103457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AKT1 antibody (381-480) [S7MR] (STJ11103457)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of lysates from 1) 3T3, 2) 293T, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-AKT1 antibody (400-480 aa) (STJ91540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-AKT1 antibody (400-480 aa) [1H2] (STJ97728)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

$64.80 - $340.20

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Anti-AKT1 antibody (60-140 aa) [1E11] (STJ97771)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of human-lung tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-AKT1 antibody (95-144 aa) (STJ91542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry with Anti-AKT1 Antibody (STJ500060) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-AKT1 antibody (C-Term) (STJ500060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Western blot analysis using Akt1 monoclonal antibody against C2C12, K562 cell lysate.

Anti-AKT1 antibody (C-Term) (STJ98461)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Cow/Chicken/Pig

$194.40 - $469.80

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Anti-AKT1 antibody (C-Term) {Biotin} (STJ500061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-AKT1 antibody (C-Term) {FITC} (STJ500062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-AKT1 antibody (N-Term) [M101] (STJA0005251)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$431.33

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Western blot detection of AKT1 in 3T3 cell lysates using AKT1 mouse mAb (dilution 1:2000) , with super ECL.Predicted band size:60KDa.Observed band size:60KDa.

Anti-AKT1 antibody [2D9-D2-H3] (STJ99156)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse/Transfected

$194.40 - $469.80

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Western blot analysis using Akt1 monoclonal antibody against NIH/3T3 (1) , HeLa (2) , COS7 (3) and Jurkat (4) cell lysate.

Anti-AKT1 antibody [3A3] (STJ97820)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Monkey

$194.40 - $469.80

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Anti-AKT1 nanobody [SAA1080] (STJN000196)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$510.98 - $2,203.88

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Anti-AKT1 nanobody [SAA1231] (STJN000197)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

IF

Reactivity :

Human

$510.98 - $2,203.88

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Western blot analysis of various lysates using ALAD Rabbit pAb (STJ110696) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ALAD antibody (1-330) (STJ110696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunofluorescence analysis of HeLa cells using ALAD antibody (STJ114271) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ALAD antibody (1-330) (STJ114271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunofluorescence analysis of HeLa cells using ALAS1 antibody (STJ115423). Blue: DAPI for nuclear staining.

Anti-ALAS1 antibody (1-250) (STJ115423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using ALAS1 antibody (STJ28604) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-ALAS1 antibody (1-250) (STJ28604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using ALAS1 Rabbit monoclonal antibody (STJ11102160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ALAS1 antibody (541-640) [S0MR] (STJ11102160)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALAS1 antibody (87-137 aa) (STJ193976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-ALAS2 antibody (434-484) (STJA0006585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using ALAS2 Rabbit polyclonal antibody (STJ28605) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALAS2 antibody (50-320) (STJ28605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates, using Albumin Rabbit pAb (STJ22574) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ALB antibody (25-609) (STJ22574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of HepG2 cells using Albumin Rabbit polyclonal antibody (STJ22575) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALB antibody (25-609) (STJ22575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-Albumin antibody at 1:2500 dilution; 10 µl of diluted human serum per lane; Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-ALB antibody (Full Length) (STJ140089)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

$134.33 - $504.23

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Western blot analysis using ALB monoclonal antibody against human albumin whole cell lysate.

Anti-ALB antibody (STJ98462)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

$194.40 - $469.80

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Western blot detection of Human Serum Albumin in 0.5nl human serum and 5ng ALB Recombinant antigens cell lysates using Human Serum Albumin mouse mAb (1:1000 diluted).Predicted band size:67KDa.Observed band size:67KDa.

Anti-ALB antibody [1A8-C5-B4] (STJ99077)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis using ALB monoclonal antibody (lane 3, 4) and Human IgG monoclonal antibody (lane 1, 2) against human serum (lane 1, 3) and plasma (lane 2, 4).

Anti-ALB antibody [2H3D1/8F6F9] (STJ97823)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot detection of Human Serum Albumin in 0.5nl human serum and 10ng ALB Recombinant antigens cell lysates using Human Serum Albumin mouse mAb (1:2000 diluted).Predicted band size:67KDa.Observed band size:67KDa.

Anti-ALB antibody [7D1-C10-E7] (STJ99078)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$194.40 - $469.80

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Anti-ALB nanobody [SAA1019] (STJN000088)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$510.98 - $2,203.88

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Anti-ALB nanobody [SAA1154] (STJN000089)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

FC

Reactivity :

Human

$510.98 - $2,203.88

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Anti-Albumin antibody [6501] (STJ400279)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$529.88 - $677.03

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Anti-Albumin antibody [6502] (STJ400008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$540.68 - $952.43

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Anti-Albumin antibody [AL-01] (STJ16100600)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/ELISA/WB

Reactivity :

Human

$547.43

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Anti-Albumin antibody [SM4927] (STJA0004927)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$315.23

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Western blot analysis of lysates from Mouse lung using Albumin/ALB Rabbit mAb (STJ11107335) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-Albumin/ALB antibody (25-609 aa) [SRM] (STJ11107335)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Anti-Albumin/HSA antibody [SAA0810] (STJA0019621)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/SPR

Reactivity :

Human

$461.03 - $1,954.13

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Anti-Albumin/HSA nanobody [SAA0807] (STJN000014)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$510.98 - $2,203.88

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Western blot analysis of lysates from Mouse lung, using Alcohol Dehydrogenase Rabbit monoclonal antibody (STJ11103058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Alcohol Dehydrogenase antibody (1-100) [S8MR] (STJ11103058)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

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Western blot analysis of mouse lung, mouse liver cells using ALDH1A1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ALDH1A1 antibody (21-70 aa) (STJ96522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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