Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of various lysates, using THBS2 Rabbit monoclonal antibody (STJ11105089) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-THBS2 antibody (19-288 aa) [S5089RM] (STJ11105089)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Western blot analysis of various lysates using THBS2 Rabbit pAb (STJ111296) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 90s.

Anti-THBS2 antibody (20-280) (STJ111296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Thioredoxin antibody western blot C6 C2C12 STJA0035417

Anti-Thioredoxin antibody [R07-4A4] (STJA0035417)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Thioredoxin antibody immunocytochemistry HeLa cells STJA0033842

Anti-Thioredoxin antibody [R07-4H5] (STJA0033842)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from Mouse pancreas, using TR Beta 1/THRB Rabbit polyclonal antibody (STJ25847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-THRB antibody (1-263) (STJ25847)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using TR Beta 1/THRB antibody (STJ11104880) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-THRB antibody (1-91) [S4880RM] (STJ11104880)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Thyroid Hormone Receptor beta Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-THRB antibody (11-60 aa) (STJ96121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-THSD7A antibody (STJA0009722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded mouse brain using CD90/Thy1 antibody (STJ119259) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-THY1 antibody (1-100) (STJ119259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using CD90/Thy1 antibody (STJ25849) at 1:700 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-THY1 antibody (20-130) (STJ25849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-THY1 antibody (51-100 aa) (STJ92148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Thyroid Hormone Receptor Beta antibody (N-Term) [2386] (STJA0003803)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£488.50

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Thyrotropin Subunit antibody immunohistochemistry pituitary adenocarcinoma STJA0033494

Anti-Thyrotropin Subunit antibody [1A1-6D9-4A7] (STJA0033494)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£214.50 - £308.50

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TSH beta antibody western blot human pituitary STJA0035485

Anti-Thyrotropin Subunit beta antibody [R13-1Z-3] (STJA0035485)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of various lysates, using TIMM10 Rabbit polyclonal antibody (STJ26764) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TIMM10 antibody (1-90) (STJ26764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TIMM10 antibody (STJA0007494)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TIMM10 antibody [SRM14791] (STJ11109521)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using TIMM17A antibody (STJ28532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TIMM17A antibody (1-171) (STJ28532)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-TIMM17A antibody (STJA0007345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using TIMM22 Rabbit monoclonal antibody (STJ11105150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TIMM22 antibody (20-120) [S5150RM] (STJ11105150)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-TIMM22 antibody (STJA0006787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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TIMM22 antibody western blot A431 cells STJA0035631

Anti-TIMM22 antibody [R47-2K-6] (STJA0035631)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-TIMM9 antibody (STJA0007168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TIMP-1 antibody [2A5] (STJ16100714)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/WB

Reactivity :

Human

£808.50

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Immunofluorescence analysis of HeLa cells using TIMP1 Rabbit polyclonal antibody (STJ28037) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TIMP1 antibody (128-207) (STJ28037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-TIMP1 (ABT-TIMP1) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 23kDaObserved band size: 23kDa

Anti-TIMP1 antibody (50-150 aa) [ABT-TIMP1] (STJ197007)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human

£180.00 - £261.00

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Human prostate tissue was stained with Anti-TIMP1 (PT2236) Antibody

Anti-TIMP1 antibody (50-150 aa) [PT2236] (STJ196989)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human

£180.00 - £261.00

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Immunofluorescence analysis of human-uterus tissue. 1, TIMP-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-TIMP1 antibody (61-110 aa) (STJ96023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Rabbit

£49.00 - £261.00

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Anti-TIMP1 antibody [2PC10] (STJA0019220)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA

Reactivity :

Human

£195.50 - £1,319.50

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Anti-TIMP1 antibody [IHC961] (STJA0042274)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-p

Reactivity :

Human

£220.50 - £706.50

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Anti-TIMP1 antibody [PT1407R] (STJA0040364)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Mouse

£150.00 - £540.00

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Anti-TIMP1 antibody [SM5019] (STJA0005019)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£228.50

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Western blot analysis of lysates from wild type (WT) and TMED10 knockout (KO) 293T cells, using [KO Validated] TMED10 Rabbit polyclonal antibody (STJ11100063) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TMED10 antibody (35-190) (STJ11100063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-TMED10 antibody (STJA0007142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of L929 cells using TMED2 Rabbit polyclonal antibody (STJ119684) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TMED2 antibody (60-170) (STJ119684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TMED2 antibody (STJA0007495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of A431 cells using TMED3 Rabbit polyclonal antibody (STJ119715) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TMED3 antibody (24-180) (STJ119715)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TMED3 antibody (STJA0007482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using TMED9 Rabbit polyclonal antibody (STJ116207) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TMED9 antibody (1-224) (STJ116207)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TMED9 antibody (STJA0007139)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TMEM132A antibody (STJA0007980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TMEM132A antibody [SAA1686] (STJA0020114)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-TMEM258 antibody (15-65 aa) (STJ196537)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of U-87MG cells using Tenascin C Rabbit polyclonal antibody (STJ11100126) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-TNC antibody (1990-2201) (STJ11100126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of KB cells using Tenascin-C Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-TNC antibody (2151-2200 aa) (STJ97243)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TNC antibody [F16] (STJA0027463)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IHC

Reactivity :

Human

£315.50

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Anti-TNC antibody [F16] {APC} (STJA0029317)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£391.50

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Anti-TNC antibody [F16] {FITC} (STJA0028351)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£315.50

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