Western blot analysis of various lysates, using TR Beta 1/THRB antibody (STJ11104880) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-THRB antibody (1-91) [S4880RM] (STJ11104880)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11104880
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General Information

Short DescriptionRabbit monoclonal Thyroid Hormone Receptor Beta (1-91) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS4880RM
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:5000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTHRB
Gene ID7068
Uniprot IDTHB_HUMAN
Immunogen Region1-91
Immunogen SequenceMTPNSMTENGLTAWDKPKHC PDREHDWKLVGMSEACLHRK SHSERRSTLKNEQSSPHLIQ TTWTSSIFHLDHDDVNDQSV SSAQTFQTEEK
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-91 of human TR Beta 1/THRB (NP_000452.2).

Additional Info

Function Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
Protein Name Thyroid Hormone Receptor Beta
Nuclear Receptor Subfamily 1 Group A Member 2
C-Erba-2
C-Erba-Beta
Database Links Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Thyroid Hormone Receptor Beta antibody
Anti-Nuclear Receptor Subfamily 1 Group A Member 2 antibody
Anti-C-Erba-2 antibody
Anti-C-Erba-Beta antibody
Anti-THRB antibody
Anti-ERBA2 antibody
Anti-NR1A2 antibody
Anti-THR1 antibody

Information sourced from Uniprot.org

Citations

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