Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of various lysates, using SLC25A5 antibody (STJ118086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-SLC25A5 antibody (199-210) (STJ118086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£110.50 - £505.50

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SLC25A5 antibody (40-120 aa) (STJ190523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of U2OS cells using SLC25A6 Rabbit polyclonal antibody (STJ25572) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SLC25A6 antibody (100-200) (STJ25572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using ANT3 Polyclonal Antibody diluted at 1:2000 STJ91606

Anti-SLC25A6 antibody (121-170 aa) (STJ91606)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SLC30A5 antibody (106-156 aa) (STJ193771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-SLC30A5 antibody (Internal Region) (STJ13100368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£322.50

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Western blot analysis of K562 cells using ZnT-8 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-SLC30A8 antibody (171-220 aa) (STJ96355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-SLC30A8 antibody (N-Term) (STJ13102152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Anti-SLC30A8 antibody [SAA1702] (STJA0020130)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Anti-SLC34A1 antibody (260-340 aa) (STJ191452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-SLC34A1 antibody (50-100 aa) (STJ13102286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Western blot analysis of various lysates, using SLC34A1 Rabbit polyclonal antibody (STJ11105721) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SLC34A1 antibody (562-639) (STJ11105721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using SLC34A1 rabbit polyclonal antibody (STJ115594) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SLC34A1 antibody (562-639) (STJ115594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using SLC34A2 Rabbit pAb (STJ111708) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-SLC34A2 antibody (234-362) (STJ111708)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SLC34A2 antibody (5-50 aa) (STJ13102287)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Western blot analysis of extracts from rat kidney, K562 cells, using NPT2b Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-SLC34A2 antibody (630-710 aa) (STJ96469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

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Anti-SLC34A2 antibody (640-690 aa) (STJ13102288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Anti-SLC34A2 antibody (STJA0038063)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£187.50 - £281.50

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Anti-SLC34A2/NaPi2b antibody [SAA0766] (STJA0027675)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/IF/IHC/WB

Reactivity :

Human

£315.50

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Anti-SLC34A2/NaPi2b antibody [SAA0766] {APC} (STJA0028926)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/IF/WB

Reactivity :

Human

£391.50

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Anti-SLC34A2/NaPi2b antibody [SAA0766] {FITC} (STJA0027960)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/IF/WB

Reactivity :

Human

£315.50

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Anti-SLC34A2/NaPi2b antibody [SAA0766] {PerCP} (STJA0029892)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/IF/WB

Reactivity :

Human

£365.50

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Anti-SLC34A2/NaPi2b antibody [SAA0766] {PE} (STJA0030858)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/IF/WB

Reactivity :

Human

£391.50

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Anti-SLC35A1 antibody (1-100) (STJ11103817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-SLC35A1 antibody (STJA0007366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from PC-12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SLC35A4 antibody (104-154 aa) (STJ195442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Smad 4 antibody [SQab22274] (STJ16103021)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£708.50

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Western blot analysis of A549, K562 cells using Smad1 polyclonal antibody. Secondary antibody was STJ96712

Anti-SMAD1 antibody (150-230 aa) (STJ96712)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 cells using Smad1 Rabbit polyclonal antibody (STJ11104508) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SMAD1 antibody (20-240) (STJ11104508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts of C6 cells, using Smad1 antibody (STJ25602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SMAD1 antibody (20-240) (STJ25602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Smad1/9 (PTR2351) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2Predicted band size: 52kDaObserved band size: 52kDa

Anti-SMAD1 antibody (400-465) [PTR2351] (STJA0006276)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Smad1 antibody [100] (STJA0032670)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£224.50 - £308.50

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Anti-Smad1 antibody [PT0687R] (STJA0039763)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Smad1 antibody western blot 293T cells STJA0036149

Anti-Smad1 antibody [R02-1J1] (STJA0036149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Smad1 antibody western blot HeLa lysates STJA0035864

Anti-Smad1 antibody [R03-4B4] (STJA0035864)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-Smad1 antibody [SRM13316] (STJ11108046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-Smad1 antibody [SRM14414] (STJ11109144)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-Smad1 antibody [SRM14522] (STJ11109252)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (STJ110012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-SMAD2 antibody (1-100) (STJ110012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Smad2 antibody (STJ11101651). Western blot was performed from the immunoprecipitate using Smad2 antibody (STJ11101651) at a dilution of 1:1000.

Anti-SMAD2 antibody (1-100) [S1MR] (STJ11101651)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded human tonsil using Smad2 Rabbit polyclonal antibody (STJ11103803) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD2 antibody (175-270) (STJ11103803)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of lysates from wild type (WT) and SMAD2 knockout (KO) Hela (KO) cells, using [KO Validated] Smad2 Rabbit monoclonal antibody (STJ11104898) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SMAD2 antibody (367-467 aa) [S4898RM] (STJ11104898)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, Smad2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). MICU1 monoclonal antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SMAD2 antibody (418-467 aa) (STJ95694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/CoIP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Smad2 antibody immunocytochemistry HeLa cells STJA0013074

Anti-SMAD2 antibody (81-100 aa) [R03-3F8] (STJA0013074)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat/Hamster

£133.50 - £281.50

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Anti-SMAD2 antibody [3C4] (STJ16103493)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/FC

Reactivity :

Human

£746.50

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Western blot analysis using Smad2 monoclonal antibody against HEK293 (1) and SMAD2-hIgGFc transfected HEK293 (2) cell lysate.

Anti-SMAD2 antibody [5G7] (STJ98383)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot detection of Smad2 in Hela, A431, Jurkat and K562 cell lysates using Smad2 mouse mAb (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Anti-SMAD2 antibody [6H5-E3-C11] (STJ99174)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£149.00 - £360.00

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Smad2 antibody western blot HeLa A431 Jurkat K562 STJA0032427

Anti-Smad2 antibody [6H5-E3-C11] (STJA0032427)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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