Haemostasis | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using Src antibody (STJ11101656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SRC antibody (1-100) [S6MR] (STJ11101656)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-SRC antibody (200-300) (STJ503200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SRC antibody (200-300) {Biotin} (STJ503202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SRC antibody (200-300) {FITC} (STJ503203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of rat-lung tissue. 1, c-Src Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-SRC antibody (360-440 aa) (STJ92501)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry of rat tissue with Anti-SRC primary antibody (STJ503201) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-SRC antibody (400-500) (STJ503201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SRC antibody (400-500) {Biotin} (STJ503204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SRC antibody (400-500) {FITC} (STJ503205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Src (PTR2316) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: HUVEC Lane 3: A431Predicted band size: 60kDaObserved band size: 60kDa

Anti-SRC antibody (400-536 aa) [PTR2316] (STJ193243)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from SH-SY5Y cells, using Src Rabbit polyclonal antibody (STJ25689) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SRC antibody (410-536) (STJ25689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SRC antibody (N-Term) [M259] (STJA0005601)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£319.50

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Immunohistochemical analysis of paraffin-embedded Human Breast CarcinomaTissue using Src mouse Monoclonal antibody diluted at 1:200.

Anti-SRC antibody [Mix] (STJ196656)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Anti-Src antibody [PT0588R] (STJA0039395)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of extracts of various cell lines, using SRGN antibody (STJ115303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-SRGN antibody (28-158) (STJ115303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SRGN antibody (30-80 aa) (STJ195103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HUVEC cells using SRGN Rabbit pAb (STJ11107044) at1:1000 dilution. <br>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection:ECL Basic Kit <br>Exposuretime:45s.

Anti-SRGN antibody (59-158 aa) (STJ11107044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded mouse heart using SRI antibody (STJ28834) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SRI antibody (1-198) (STJ28834)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-SRI antibody (STJA0007305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of Stromal interaction molecule 1 in MCF-7, C6, 3T3, Hela lysates using Stromal Interaction Molecule 1 antibody.

Anti-STIM1 antibody (199-212 aa) [R07-9F9] (STJA0012009)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£264.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g STIM1 antibody (STJ11104440). Western blot was performed from the immunoprecipitate using STIM1 antibody (STJ11104440) at a dilution of 1:1000.

Anti-STIM1 antibody (451-685) (STJ11104440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using STIM1 Rabbit mAb (STJ11106983) at 1:2000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-STIM1 antibody (530-685 aa) [SRM5145] (STJ11106983)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Anti-STIM1 antibody (530-685 aa) [SRM5163] (STJ11107017)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of extracts of various cell lines, using STIM1 antibody (STJ11100938) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-STIM1 antibody (530-685) (STJ11100938)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Rat testis, using STIM1 Rabbit mAb (STJ11103679) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-STIM1 antibody (586-685) [S9MR] (STJ11103679)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-STIM1 antibody (Cytoplasmic Domain) (STJ13100341)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse/Marmoset

£331.50

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Anti-STIM1 antibody (ECD) (STJ13102084)

Clonality :

Polyclonal

Host :

Goat

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-STIM1 antibody (N-Term) (STJA0010329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-STIM1 antibody [SAA1741] (STJA0020169)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Anti-STIM1 antibody [SRM13242] (STJ11107972)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£0.00

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Anti-STX4 antibody (STJA0007007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using STXBP2 antibody (STJ110046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-STXBP2 antibody (1-280) (STJ110046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using STXBP2 antibody (STJ114385) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-STXBP2 antibody (1-280) (STJ114385)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-STXBP2 antibody (392-442 aa) (STJ196125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using STXBP3 Rabbit pAb (STJ110452) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-STXBP3 antibody (343-592) (STJ110452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-STXBP3 antibody (STJA0007511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot with Anti-SYK Antibody (STJ503182) on mouse bladder. Antibody at 1:500 dilution in buffer.

Anti-SYK antibody (100-150) (STJ503182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SYK antibody (100-150) {Biotin} (STJ503183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SYK antibody (100-150) {FITC} (STJ503184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of U2OS using Syk antibody (STJ25758) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SYK antibody (250-350) (STJ25758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SYK antibody (290-370 aa) (STJ95845)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-SYK antibody (Central region) [M373] (STJA0005609)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot analysis of CACO2 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-SYK antibody (STJ99608)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Syk antibody [PT0468R] (STJA0039492)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Anti-SYK antibody [SAA1636] (STJA0020064)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Anti-Syk antibody [SRM14840] (STJ11109570)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IF/ICC/IF-P/FC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Syk antibody [SYK-01] (STJ16100208)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IP/WB/IHC-P/ICC

Reactivity :

Human/Rat/Mouse

£537.50

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Anti-Syndecan 4 antibody {ABflo® 647} (STJ11108901)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

£0.00

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Immunofluorescence analysis of NIH-3T3 cells using Syntaxin 4 Rabbit monoclonal antibody (STJ11103604) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Syntaxin 4 antibody (50-150) [S4MR] (STJ11103604)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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