Anti-Profilin 1 antibody [R06-6H7] (STJA0033866)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human Profilin 1
General Information
| Short Description | Rabbit monoclonal anti-Profilin 1 for use in WB, IHC-F, IHC-P, ICC, IF and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R06-6H7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | PFN1 |
| Gene ID | 5216 |
| Uniprot ID | PROF1_HUMAN |
| Immunogen | A synthetic peptide of human Profilin 1 |
Additional Info
| Tissue Specificity | Expressed in epididymis (at protein level). |
| Post Translational Modifications | Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation. |
| Function | Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR. |
| Protein Name | Profilin-1Epididymis Tissue Protein Li 184aProfilin I |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-376176Reactome: R-HSA-4086400Reactome: R-HSA-5663220 |
| Cellular Localisation | CytoplasmCytoskeleton |
| Alternative Antibody Names | Anti-Profilin-1 antibodyAnti-Epididymis Tissue Protein Li 184a antibodyAnti-Profilin I antibodyAnti-PFN1 antibody |
Information sourced from Uniprot.org