Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis using NF Kappa B-p105/p50 monoclonal antibody against K562 (1) , Jurkat (2) , A431 (3) , HeLa (4) , THP-1 (5) and MCF-7 (6) cell lysate.

Anti-NFKB1 antibody [5D10D11] (STJ98274)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot detection of NF-Kappa B1 p105/p50 in K562, C6, 3T3 and Hela cell lysates using NF-Kappa B1 p105/p50 mouse mAb (dilution 1:500).Predicted band size:120, 50kDa.Observed band size:120, 50kDa.

Anti-NFKB1 antibody [5E3-E4-C11] (STJ99314)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of Hela cell. 1, NF-YA Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3A mouse monoclonal antibody (5G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-NFYA antibody (261-310) (STJ94455)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using NFYB antibody (STJ29434) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NFYB antibody (1-207) (STJ29434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using NF-YB Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NFYB antibody (1-50) (STJ94456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using NF-YC Polyclonal Antibody diluted at 1：1000

Anti-NFYC antibody (1-50) (STJ94457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Rat thymus, using NFYC Rabbit pAb (STJ111874) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-NFYC antibody (27-335) (STJ111874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using NKX2-5 antibody (STJ27618) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.

Anti-NKX2-5 antibody (1-135) (STJ27618)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using Nkx-2.5 monoclonal antibody against full-length NKX2.5 (aa1-324) -hIgGFc transfected HEK293 cell lysate (1).

Anti-NKX2-5 antibody [2E1] (STJ98278)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with NKX3-2, using NKX3-2 Rabbit pAb (STJ111268) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-NKX3-2 antibody (1-180) (STJ111268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NKX3-2 antibody (STJA0008774)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NLRC4 antibody (481-531 aa) (STJ195643)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-NLRC4 antibody (85-170) (STJ29519)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NLRC4 antibody (N-Term) (STJA0005739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£455.50

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-NOC2L antibody (602-651) (STJ94525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-NOC2L antibody (650-749 aa) (STJ11106423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from A-549 cells, using Non-phospho-YAP1-S127 Rabbit pAb (STJ11103239) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Non-phospho-YAP1-S127 antibody (50-150) (STJ11103239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using NOP2 antibody (STJ11103028) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NOP2 antibody (355-590) (STJ11103028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-NOP2 antibody (STJA0007573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of extracts of Jurkat cells, using Notch1 antibody (STJ11100238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NOTCH1 antibody (1567-1729) (STJ11100238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using Notch1 Rabbit pAb (STJ119092) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-NOTCH1 antibody (1715-1814) (STJ119092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using Notch1 Rabbit polyclonal antibody (STJ11103863) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOTCH1 antibody (2381-2480) (STJ11103863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Rat brain cells, using Notch1 antibody (STJ29951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NOTCH1 antibody (2456-2555) (STJ29951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g Notch1 antibody (STJ11101627). Western blot was performed from the immunoprecipitate using Notch1 antibody (STJ11101627) at a dilution of 1:1000.

Anti-NOTCH1 antibody (2456-2555) [S7MR] (STJ11101627)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of extracts of U-251MG cells, using Notch1 antibody (STJ119090) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.

Anti-NOTCH1 antibody (2504-2518) (STJ119090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NOTCH1 antibody [SAA0131] (STJA0026984)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£254.50 - £378.50

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Anti-NOTCH1 antibody [SAA0131] {APC} (STJA0029442)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£310.50 - £472.50

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Anti-NOTCH1 antibody [SAA0131] {FITC} (STJA0028476)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£254.50 - £378.50

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Anti-NOTCH1 antibody [SAA0131] {PerCP} (STJA0030408)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£292.50 - £440.50

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Anti-NOTCH1 antibody [SAA0131] {PE} (STJA0031374)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£310.50 - £472.50

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Anti-NOTCH1 nanobody [SAA1286] (STJN000228)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Immunofluorescence analysis of C6 cells using NOTCH2 Rabbit polyclonal antibody (STJ11105517) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOTCH2 antibody (1697-1819) (STJ11105517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using NOTCH2 antibody (STJ29808) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NOTCH2 antibody (2242-2471) (STJ29808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-NOTCH2 nanobody [SAA1287] (STJN000257)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Immunofluorescence analysis of U2OS cells using NOTCH3 Rabbit polyclonal antibody (STJ24789) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOTCH3 antibody (1520-1640) (STJ24789)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NOTCH3 antibody (2222-2321 aa) [SRM] (STJ11107194)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded mouse pancreas using NOTCH3 antibody (STJ115483) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NOTCH3 antibody (2305-2321) (STJ115483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using NOTCH3 Rabbit polyclonal antibody (STJ24788) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NOTCH3 antibody (751-850) (STJ24788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-NOTCH3 antibody (C-Term) (STJA0005815)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human

£455.50

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Anti-NOTCH3 nanobody [SAA1288] (STJN000313)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of lysates from Mouse lung, using NOTCH4 Rabbit pAb (STJ110601) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-NOTCH4 antibody (1824-2003) (STJ110601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NOTCH4 antibody [SAA1712] (STJA0020140)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NPAS4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-NPAS4 antibody (603-652) (STJ94544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-NPAS4 antibody (STJ501951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-NPAS4 antibody {Biotin} (STJ501952)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-NPAS4 antibody {FITC} (STJ501953)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Nucleophosmin antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-NPM1 antibody (1-19 aa) [R06-4G7] (STJA0011706)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£246.50

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Western blot analysis of various cells using Nucleophosmin Polyclonal Antibody diluted at 1：2000

Anti-NPM1 antibody (1-50) (STJ94573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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