Anti-HSL/LIPE antibody [SRM14764] (STJ11109494)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal HSL/LIPE antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14764 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:8000-1:32000IF/ICC, 1:50-1:200ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LIPE |
| Gene ID | 3991 |
| Uniprot ID | LIPS_HUMAN |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | Testis. |
| Post Translational Modifications | Phosphorylation by AMPK reduces its translocation towards the lipid droplets. |
| Function | Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. |
| Protein Name | Hormone-Sensitive LipaseHslMonoacylglycerol Lipase LipeRetinyl Ester HydrolaseReh |
| Database Links | Reactome: R-HSA-163560Reactome: R-HSA-9841922 |
| Cellular Localisation | Cell MembraneMembraneCaveolaCytoplasmCytosolLipid DropletFound In The High-Density CaveolaeTranslocates To The Cytoplasm From The Caveolae Upon Insulin StimulationPhosphorylation By Ampk Reduces Its Translocation Towards The Lipid Droplets |
| Alternative Antibody Names | Anti-Hormone-Sensitive Lipase antibodyAnti-Hsl antibodyAnti-Monoacylglycerol Lipase Lipe antibodyAnti-Retinyl Ester Hydrolase antibodyAnti-Reh antibodyAnti-LIPE antibody |
Information sourced from Uniprot.org