Anti-HP1 gamma antibody [5G10-F7-A12] (STJA0033180)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant human HP1-gamma protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-HP1 gamma for use in WB, IHC-F, IHC-P, ICC, IF and IP in Mouse, Human, Monkey, Hamster and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF/IP |
| Host | Mouse |
| Reactivity | Mouse/Human/Monkey/Hamster/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 5G10-F7-A12 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CBX3 |
| Gene ID | 11335 |
| Uniprot ID | CBX3_HUMAN |
| Immunogen | Purified recombinant human HP1-gamma protein fragments expressed in E.coli. |
Additional Info
| Post Translational Modifications | Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. |
| Function | Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs). |
| Protein Name | Chromobox Protein Homolog 3HechHeterochromatin Protein 1 Homolog GammaHp1 GammaModifier 2 Protein |
| Database Links | Reactome: R-HSA-427389Reactome: R-HSA-73772Reactome: R-HSA-8953750 |
| Cellular Localisation | NucleusAssociates With Euchromatin And Is Largely Excluded From Constitutive HeterochromatinMay Be Associated With Microtubules And Mitotic Poles During Mitosis (Potential) |
| Alternative Antibody Names | Anti-Chromobox Protein Homolog 3 antibodyAnti-Hech antibodyAnti-Heterochromatin Protein 1 Homolog Gamma antibodyAnti-Hp1 Gamma antibodyAnti-Modifier 2 Protein antibodyAnti-CBX3 antibody |
Information sourced from Uniprot.org