• Western blot analysis of mouse large intestine, using HNF4G antibody (STJ118492) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of Rat liver, using HNF4G antibody (STJ118492) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of U-2 OS cells using HNF4G Polyclonal Antibody (STJ118492) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HNF4G antibody (366-445) (STJ118492)

SKU:
STJ118492

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HNF4G (366-445) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNF4G
Gene ID: 3174
Uniprot ID: HNF4G_HUMAN
Immunogen Region: 366-445
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 366-445 of human HNF4G (NP_004124.4?).
Immunogen Sequence: ASNDGSHLHHPMHPHLSQDP LTGQTILLGPMSTLVHADQI STPETPLPSPPQGSGQEQYK IAANQASVISHQHLSKQKQL
Tissue Specificity Expressed in pancreas, kidney, small intestine and testis. Weakly expressed in colon. Not expressed in liver, skeletal muscle, lung, placenta, brain, heart, peripheral blood, ovary, prostate, thymus and spleen.
Function Transcription factor. Has a lower transcription activation potential than HNF4-alpha.
Protein Name Hepatocyte Nuclear Factor 4-Gamma
Hnf-4-Gamma
Nuclear Receptor Subfamily 2 Group A Member 2
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-383280
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hepatocyte Nuclear Factor 4-Gamma antibody
Anti-Hnf-4-Gamma antibody
Anti-Nuclear Receptor Subfamily 2 Group A Member 2 antibody
Anti-HNF4G antibody
Anti-NR2A2 antibody

Information sourced from Uniprot.org

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