Gene Expression And Transcription | Research Area | St John's Laboratory

Rat LV-Htt171-82Q (800 ng) , PFA 4%, HTT antibody, 1:2000 PBS-BSA 1% – 25µm

Anti-HTT antibody (85 to 200aa) (STJ140045)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Immunohistochemistry analysis of paraffin-embedded human uterine cancer using HUS1 antibody (STJ115873) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HUS1 antibody (1-280) (STJ115873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HUS1 antibody (1-280) (STJ27360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HUS1 antibody (160-240 aa) (STJ190415)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-ICE2 antibody (474-524) (STJA0006645)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of mouse brain, using ICE2 antibody (STJ114737) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ICE2 antibody (660-800) (STJ114737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Anti-IFN-gamma antibody (STJ16101138)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-F/ELISA/WB

Reactivity :

Rat

£662.50

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Anti-IFN-Gamma antibody [B27] {EF700} (STJA0016464)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £331.50

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Anti-IFN-gamma antibody [F14] (STJ16100661)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/ELISA/IP

Reactivity :

Human

£525.50

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Anti-IFN-gamma antibody [F1] (STJ16100652)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IP

Reactivity :

Mouse

£460.50

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Anti-IFN-gamma antibody [F3] (STJ16100653)

Clonality :

Monoclonal

Host :

Rat

Applications :

FUNC/ELISA/IP

Reactivity :

Mouse

£460.50

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Western blot analysis of various cells using IFN-Gamma Polyclonal Antibody diluted at 1：1000

Anti-IFNG antibody (20-69 aa) (STJ93644)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of IFN gamma in paraffin-embedded human lung using IFN gamma Rabbit polyclonal antibody (STJ114324) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-IFNG antibody (24-161) (STJ114324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of recombinant Human IFN gamma Protein, using IFN gamma Rabbit monoclonal antibody (STJ11105259) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-IFNG antibody (24-161) [S5259RM] (STJ11105259)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of recombinant Human Interferon Gamma Protein using IFN gamma Rabbit polyclonal antibody (STJ24133) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-IFNG antibody (24-515) (STJ24133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis using IFN-Gamma monoclonal antibody against IFN-Gamma recombinant protein.

Anti-IFNG antibody [1B1A4/E8H1] (STJ98156)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-IFNG antibody [B27] (STJA0002120)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICFC/WB/IHC/IP

Reactivity :

Human

£93.50 - £188.50

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Anti-IFNG antibody [B27] (STJA0002121)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICFC/WB/IHC/IP

Reactivity :

Human

£93.50 - £114.50

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Anti-IFNG antibody [B27] {APC} (STJA0002124)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICFC

Reactivity :

Human

£142.50 - £357.50

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Anti-IFNG antibody [B27] {FITC} (STJA0002122)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICFC

Reactivity :

Human

£131.50 - £319.50

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Anti-IFNG antibody [B27] {PE} (STJA0002123)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICFC

Reactivity :

Human

£131.50 - £319.50

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Western blot analysis of extracts of HepG2 cells, using IGFBP1 antibody (STJ113709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IGFBP1 antibody (150-259) (STJ113709)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of Mouse liver, using IGFBP1 antibody (STJ11101385) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-IGFBP1 antibody (160-259) [S5MR] (STJ11101385)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of extracts of HepG2 cells, using IGFBP1 antibody (STJ24145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IGFBP1 antibody (81-259) (STJ24145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of IGFBP1 in Human placenta lysates using IGFBP1 antibody.

Anti-IGFBP1 antibody (88-100 aa) [R06-6J8] (STJA0011477)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£246.50

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Western blot analysis of MCF7 cells using IGFBP1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IGFBP1 antibody (91-140 aa) (STJ97262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using IGFBP3 antibody (STJ118493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IGFBP3 antibody (100-200) (STJ118493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using IGFBP3 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-IGFBP3 antibody (151-200 aa) (STJ93651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using IHH antibody (STJ28709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IHH antibody (202-411) (STJ28709)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts from NIH-3T3 cells, using Ihh Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IHH antibody (209-258 aa) (STJ96388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-IL-6 antibody [2707] (STJ400294)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£507.50 - £659.50

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Anti-IL-6 antibody [MP5-20F3] (STJ16100654)

Clonality :

Monoclonal

Host :

Rat

Applications :

FUNC/ELISA/IP

Reactivity :

Mouse

£460.50

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Flow cytometry was performed with Jurkat cells.

Anti-IL2 antibody (1-153) [S3947RM] (STJ11103947)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various cells using IL-2 Polyclonal Antibody

Anti-IL2 antibody (16-65 aa) (STJ93688)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using IL2 antibody (STJ118747) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-IL2 antibody (21-153) (STJ118747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of recombinant Human IL2 Protein, using IL2 Rabbit polyclonal antibody (STJ24180) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 500pg/250pg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-IL2 antibody (21-153) (STJ24180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of recombinant Human IL-2 Protein, using IL2 Rabbit monoclonal antibody (STJ11105271) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 1ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IL2 antibody (21-153) [S5271RM] (STJ11105271)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis using IL-2 monoclonal antibody against full-length IL-2 recombinant protein with Trx tag (1) and full-length IL-2-hIgGFc transfected HEK293 cell lysate (2).

Anti-IL2 antibody [8D5] (STJ98172)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-IL2 antibody [MQ1-17H12] {APC} (STJA0002141)

Clonality :

Monoclonal

Host :

Rat

Applications :

ICFC

Reactivity :

Human

£142.50 - £310.50

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Anti-IL2 antibody [MQ1-17H12] {FITC} (STJA0002139)

Clonality :

Monoclonal

Host :

Rat

Applications :

ICFC

Reactivity :

Human

£131.50 - £279.50

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Anti-IL2 antibody [MQ1-17H12] {PE} (STJA0002140)

Clonality :

Monoclonal

Host :

Rat

Applications :

ICFC

Reactivity :

Human

£131.50 - £279.50

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Western blot analysis of extracts of various cell lines, using CD25 antibody (STJ24183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IL2RA antibody (153-167) (STJ24183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells, using IL-2R alpha/CD25 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-IL2RA antibody (223-272 aa) (STJ93693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, CD25 monoclonal antibody (Q22) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IL2RA antibody [Q22] (STJ97009)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using IL3 antibody (STJ27638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IL3 antibody (20-152) (STJ27638)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-IL3 antibody (STJA0010239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using IL6 antibody (STJ111153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL6 antibody (1-100) (STJ111153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from HUVEC cells, using IL6 Rabbit monoclonal antibody (STJ11104756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-IL6 antibody (1-212) [S4756RM] (STJ11104756)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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