Anti-Cyclin D3/CCND3 antibody (STJA0037896)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Cyclin D3
General Information
| Short Description | Rabbit monoclonal anti-Cyclin D3/CCND3 for use in IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | IHC 1:100-1:200 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CCND3 |
| Gene ID | 896 |
| Uniprot ID | CCND3_HUMAN |
| Immunogen | A synthesized peptide derived from human Cyclin D3 |
Additional Info
| Post Translational Modifications | Phosphorylation at Thr-283 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex. Ubiquitinated by the DCX(AMBRA1) complex during the transition from G1 to S cell phase, leading to its degradation: ubiquitination is dependent on Thr-283 phosphorylation. The DCX(AMBRA1) complex represents the major regulator of CCND3 stability during the G1/S transition. Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation. |
| Function | Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Shows transcriptional coactivator activity with ATF5 independently of CDK4. |
| Protein Name | G1/S-Specific Cyclin-D3 |
| Database Links | Reactome: R-HSA-381340Reactome: R-HSA-5687128Reactome: R-HSA-69231Reactome: R-HSA-8934593Reactome: R-HSA-9661069Reactome: R-HSA-9754119 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-G1/S-Specific Cyclin-D3 antibodyAnti-CCND3 antibody |
Information sourced from Uniprot.org