Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of lysates from wild type (WT) and UHRF1 knockdown (KD) 293T cells using [KD Validated] UHRF1 Rabbit pAb (STJ11107094) at 1:1000 dilution incubated overnight at 4°C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit (

Anti-UHRF1 antibody (672-793 aa) (STJ11107094)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of extracts from Jurkat cells, using UHRF1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-UHRF1 antibody (Internal) (STJ96431)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western Blot analysis using UHRF1 Monoclonal Antibody against mouse heart, 293T, HeLa cell lysate, HeLa nuclear extract.

Anti-UHRF1 antibody (N-Term) (STJ98567)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human

€180.00 - €435.00

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Western blot detection of UHRF1 in Mouse heart, 293T, HelaNE and Hela cell lysates using UHRF1 mouse mAb (1:1000 diluted).Predicted band size: 90KDa.Observed band size: 90KDa.

Anti-UHRF1 antibody [1H9-D3-E11] (STJ99024)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse

€180.00 - €435.00

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Anti-UPF3B antibody (STJA0007940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€60.00 - €315.00

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Anti-USP2 antibody (452-502) (STJA0006460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using USP7/HAUSP rabbit monoclonal antibody (STJ11101871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-USP7 antibody (100-200) [S1MR] (STJ11101871)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Immunofluorescence analysis of HeLa cells using USP7/HAUSP antibody (STJ115525).

Anti-USP7 antibody (206-1102) (STJ115525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using USP7/HAUSP Rabbit polyclonal antibody (STJ26064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-USP7 antibody (206-1102) (STJ26064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis using HAUSP monoclonal antibody against HeLa cell lysate.

Anti-USP7 antibody (C-Term) (STJ98495)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Dog/Pig

€180.00 - €435.00

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Western blot detection of HAUSP/USP7 in Hela and K562 cell lysates using HAUSP/USP7 mouse mAb (1:200 diluted) , with super ECL.Predicted band size:135KDa.Observed band size:135KDa.

Anti-USP7 antibody [3E4-D10-E8] (STJ99076)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

€180.00 - €435.00

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Western blot analysis of various lysates using USP9X Rabbit polyclonal antibody (STJ118737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.

Anti-USP9X antibody (1-100) (STJ118737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using USP9X Rabbit mAb (STJ11102548) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-USP9X antibody (1-100) [S8MR] (STJ11102548)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Anti-USP9X antibody (STJA0006795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-UXT antibody (STJA0008017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using VDR antibody (STJ26084) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-VDR antibody (1-100) (STJ26084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using VDR antibody (STJ113337) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-VDR antibody (1-300) (STJ113337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using VDR antibody (STJ11104082) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-VDR antibody (1-300) [S4082RM] (STJ11104082)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€161.88 - €718.13

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Whole cell lysates of Hela were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Vitamin D Receptor (PTR2542) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 48kDaObserved band size: 37kDa

Anti-VDR antibody (150-250) [PTR2542] (STJA0006257)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA/ELISA

Reactivity :

Human

€60.00 - €315.00

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Western blot analysis of various cells using VDR Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-VDR antibody (181-230 aa) (STJ96233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of NIH/3T3 cells using VEGFA Rabbit polyclonal antibody (STJ27525) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-VEGFA antibody (100-200) (STJ27525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-VEGF-A (PT0865) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: LnCapPredicted band size: 27kDaObserved band size: 42kDa

Anti-VEGFA antibody (100-200) [PT0865] (STJA0006227)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

€60.00 - €315.00

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Immunofluorescence analysis of mouse-kidney tissue. 1, VEGF-A Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-VEGFA antibody (110-159 aa) (STJ96235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Pig/Rabbit

€60.00 - €315.00

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Western blot analysis of various lysates, using VEGFA Mouse monoclonal antibody (STJ119887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-VEGFA antibody (207-327) [S7MM] (STJ119887)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€161.88 - €718.13

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Immunofluorescence analysis of NIH/3T3 cells using VEGFA Rabbit polyclonal antibody (STJ114190) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Anti-VEGFA antibody (27-126) (STJ114190)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of NIH/3T3 using VEGF antibody (STJ11104459) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-VEGFA antibody (51-327) (STJ11104459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using VEGFA antibody (STJ29920) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-VEGFA antibody (68-147) (STJ29920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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MBP-VEGFA isoform 6 recombinant protein detected by western blot using anti-VEGFA antibody at 1000 dilution; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-VEGFA antibody (STJ140053)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€124.38 - €466.88

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Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using VEGF Rabbit pAb diluted at 1:500.

Anti-VEGFA antibody (STJ197324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-VEGFA antibody [SM5189] (STJA0005189)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

€291.88

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Western blot analysis of extracts of various cell lines, using VENTX antibody (STJ29603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-VENTX antibody (1-258) (STJ29603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-WDR33 antibody (319-369 aa) (STJ194468)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of HeLa cells, using WDR5 antibody (STJ115543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-WDR5 antibody (1-334) (STJ115543)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Immunohistochemistry of baboon kidney with Anti-WDR5 primary antibody (STJ503518) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-WDR5 antibody (157-174) (STJ503518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

A.thaliana/Bovine/Chicken/Dog/Human/Monkey/Mouse/Pig/Rat/Xenopus/Zebra Fish

€534.38

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Anti-WDR5 antibody (157-174) {Biotin} (STJ503519)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€660.63

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Anti-WDR5 antibody (157-174) {FITC} (STJ503520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€660.63

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Western blot analysis of extracts of various cell lines, using WDR5 Rabbit monoclonal antibody (STJ11101844) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-WDR5 antibody (235-334) [S4MR] (STJ11101844)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€161.88 - €718.13

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Western blot analysis using WDR5 monoclonal antibody against HeLa (1) cell lysate.

Anti-WDR5 antibody [7B11] (STJ98449)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

€180.00 - €435.00

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Immunohistochemistry analysis of WDR61 in paraffin-embedded Rat brain using WDR61 Rabbit polyclonal antibody (STJ117715) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-WDR61 antibody (1-305) (STJ117715)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-WDR82 antibody (219-269 aa) (STJ194690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-WRN antibody (1080-1160) (STJA0010091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using WRN antibody (STJ28937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-WRN antibody (1223-1432) (STJ28937)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Anti-WWOX 1 + 4 antibody (STJ503537)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse

€583.13

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Anti-WWOX 1 + 4 antibody {Biotin} (STJ503538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse

€660.63

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Anti-WWOX 1 + 4 antibody {FITC} (STJ503539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse

€660.63

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Immunofluorescence analysis of NIH-3T3 cells using WWOX Rabbit polyclonal antibody (STJ114526) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-WWOX antibody (1-120) (STJ114526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of various lysates using WWOX Rabbit polyclonal antibody (STJ114527) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-WWOX antibody (1-120) (STJ114527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from HepG2 cells, using WWOX Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-WWOX antibody (1-50 aa) (STJ96274)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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