DNA Repair | Research Area | St John's Laboratory

Western blot analysis of lysates from HUVEC cells treated with Forskolin 40nM 30', using Nibrin (Phospho-Ser278) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-NBN-Ser278 antibody (251-300 aa) (STJ90727)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells, using p95/NBS1 (Phospho-Ser343) Antibody. The lane on the left is blocked with the phospho peptide.

Anti-Phospho-NBN-Ser343 antibody (310-359 aa) (STJ90354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Anti-Phospho-NBN-Ser343 antibody (STJ196375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-NBS1/NBN-S343 antibody (STJ11107709)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-p53-S20 antibody (STJ11107670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-p53-Ser15 antibody [PT0901R] (STJA0039935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-p53-Ser392 antibody [PT1223R] (STJA0040171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-p53-Ser46 antibody [PT0903R] (STJA0039796)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-p53-Ser6 antibody [PT1249R] (STJA0040157)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-p53-T18 antibody [23#] (STJA0019168)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of lysates from C6 cells, using Phospho-p53-T81 Rabbit pAb (STJ11103770) at 1:500 dilution. C6 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-p53-T81 antibody (STJ11103770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PNKP-Ser114/Thr118 antibody (STJ196388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-POLR2A-CTD antibody (YSPTSPSYSPTSPS) [1stS2] (STJA0019158)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-POLR2A-CTD antibody (YSPTSPSYSPTSPS) [1stS5] (STJA0019159)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-POLR2A-CTD antibody (YSPTSPSYSPTSPS) [2ndS2] (STJA0019161)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-POLR2A-CTD antibody (YSPTSPSYSPTSPS) [S7] (STJA0019160)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-POLR2A-CTD antibody (YSPTSPSYSPTSPS) [S7S5] (STJA0019162)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of lysates from 293T cells, using Phospho-POLR2A CTD-S2 Rabbit pAb (STJ116378) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour or treated by Doxorubicin (0. 5uM) for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S2 antibody (STJ116378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£129.50 - £642.50

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Chromatin immunoprecipitations were performed with cross-linked chromatin from 293F cells and Phospho-POLR2A-S2 (STJ11102585). The ChIP sequencing results indicate the enrichment pattern of Phospho-POLR2A-S2 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Phospho-POLR2A-S2 antibody [S5MR] (STJ11102585)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq/CUT&Tag

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of HeLa, using Phospho-POLR2A CTD-S2/S5 antibody (STJ11105825) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-Phospho-POLR2A-S2/S5 antibody (STJ11105825)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit pAb (STJ117926) at 1:1000 dilution. MCF7 and C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S5 antibody (STJ117926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb (STJ11102586) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours or treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by CIP (20uL/400ul) at 37 °C for 1 hour. C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S5 antibody [S6MR] (STJ11102586)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-POLR2A CTD-S7 Rabbit pAb (STJ11103724) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours. MCF7 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S7 antibody (STJ11103724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of VEC using p-Rpb1 (S1619) antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-POLR2A-Ser1619 antibody (1585-1634 aa) (STJ91031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-POLR2A-Ser2 antibody (STJ196405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-POLR2A-Ser5 antibody (STJ196406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-POLR2A-Ser7 antibody (STJ196407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Chromatin immunoprecipitation analysis of extracts of 293T; cells, using Phospho-POLR2A CTD-T4 antibody (STJ11104227) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Phospho-POLR2A-T4 antibody (STJ11104227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates, using Phospho-POLR2A-T4 monoclonal antibody antibody (STJ11105859) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-POLR2A-T4 antibody [S5859RM] (STJ11105859)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-POLR2A-Thr4 antibody (STJ196408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-POLR2A-Thr4 antibody (STJ196409)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-DNA-PKcs-S2056 Rabbit pAb (STJ113503). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PRKDC-S2056 antibody (STJ113503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DNA-PK (Phospho-Ser2056) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PRKDC-Ser2056 antibody (2022-2071 aa) (STJ91147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-PRKDC-Ser2612 antibody (2578-2627 aa) (STJ91146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-PRKDC-Thr2609 antibody (STJA0009815)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using DNA-PK (Phospho-Thr2638) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PRKDC-Thr2638 antibody (2604-2653 aa) (STJ91334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using DNA-PK (Phospho-Thr2647) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PRKDC-Thr2647 antibody (2613-2662 aa) (STJ91305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RAD17-Ser635 antibody (STJ196393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Rad17 (S645) Polyclonal Antibody

Anti-Phospho-RAD17-Ser645 antibody (621-670 aa) (STJ90451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts from K562 cells, using Phospho-Rad17 (S646) Polyclonal Antibody.

Anti-Phospho-RAD17-Ser646 antibody (STJ91363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RAD18-Ser403 antibody (STJ196394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RAD50-Ser635 antibody (STJ196395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of HELA-UV 293T-UV using p-Rad51 (T309) antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-RAD51-Thr309 antibody (275-324 aa) (STJ91266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells, using RAD51 (Phospho-Tyr315) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RAD51-Tyr315 antibody (281-330 aa) (STJ90536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30', using RAD52 (Phospho-Tyr104) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RAD52-Tyr104 antibody (70-119 aa) (STJ90918)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Western blot of human T47D cell lysate showing specific immunolabeling of the ~100 kDa CtIP phosphorylated at Ser326 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-RBBP8-Ser326 antibody (STJA0003827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

£454.50

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Western blot of human T47D cell lysate showing specific immunolabeling of the ~100 kDa CtIP phosphorylated at Ser327 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-RBBP8-Ser327 antibody (STJA0003828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

£454.50

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Anti-Phospho-RBBP8-Ser327 antibody (STJA0009814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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