| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PARG (777-976) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PARG |
| Gene ID: |
670/8505 |
| Uniprot ID: |
PARG_HUMAN |
| Immunogen Region: |
777-976 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 777-976 of human PARG (NP_003622.2). |
| Immunogen Sequence: |
HNECLIITGTEQYSEYTGYA ETYRWSRSHEDGSERDDWQR RCTEIVAIDALHFRRYLDQF VPEKMRRELNKAYCGFLRPG VSSENLSAVATGNWGCGAFG GDARLKALIQILAAAAAERD VVYFTFGDSELMRDIYSMHI FLTERKLTVGDVYKLLLRYY NEECRNCSTPGPDIKLYPFI YHAVESCAETADHSGQRTGT |
| Tissue Specificity | Ubiquitously expressed. |
| Function | Poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose). PARG acts both as an endo- and exoglycosidase, releasing poly(ADP-ribose) of different length as well as ADP-ribose monomers. It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated. Poly(ADP-ribose) is synthesized after DNA damage is only present transiently and is rapidly degraded by PARG. Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress. Responsible for the prevalence of mono-ADP-ribosylated proteins in cells, thanks to its ability to degrade poly(ADP-ribose) without cleaving the terminal protein-ribose bond. Required for retinoid acid-dependent gene transactivation, probably by removing poly(ADP-ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR-dependent gene promoters. Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming. |
| Protein Name | Poly(Adp-Ribose Glycohydrolase |
| Database Links | Reactome: R-HSA-110362 |
| Cellular Localisation | Isoform 1: NucleusColocalizes With Pcna At Replication FociRelocalizes To The Cytoplasm In Response To Dna DamageIsoform 2: CytoplasmTranslocates To The Nucleus In Response To Dna DamageIsoform 3: CytoplasmIsoform 4: CytoplasmMitochondrionIsoform 5: Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Poly(Adp-Ribose Glycohydrolase antibodyAnti-PARG antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
