Disease | Research Area | St John's Laboratory

Western blot analysis of lysates from 293T cells, using Phospho-mTOR-S2448 Rabbit mAb (STJ11102554) at 1:1000 dilution. 293T cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight or treated by IGF-1 (50 ng/mL) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-mTOR-S2448 antibody [S4MR] (STJ11102554)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€172.20 - €742.20

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Western blot analysis of lysates from C6 cells using Phospho-mTOR-S2481 Rabbit pAb (STJ11102557) at 1:2000 dilution. C6 cells were treated by IGF-1 (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-mTOR-S2481 antibody (STJ11102557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of various lysates using Phospho-mTOR-S2481 (STJ11102570) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by IGF-1 (50 ng/mL) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-MTOR-S2481 antibody [S0MR] (STJ11102570)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€142.20 - €625.80

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Immunofluorescence analysis of Hela cell. 1, mTOR (phospho Ser2448) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MTOR-Ser2448 antibody (2415-2464 aa) (STJ90336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Cow/Pig

€58.80 - €313.20

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Anti-Phospho-mTOR-Ser2448 antibody [PT0498R] (STJA0039463)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Phospho-mTOR Ser2448 antibody western blot HeLa cells STJA0034589

Anti-Phospho-mTOR-Ser2448 antibody [R08-7G7] (STJA0034589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse

€160.20 - €337.80

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Phospho-mTOR antibody western blot HEK293 lysates STJA0035671

Anti-Phospho-mTOR-Ser2448 antibody [R13-9D-7] (STJA0035671)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat/Pig

€160.20 - €337.80

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Western blot analysis of hela-UV using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-MTOR-Ser2481 antibody (2447-2496 aa) (STJ91294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Cow

€58.80 - €313.20

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Anti-Phospho-mTOR-Ser2481 antibody [PT0898R] (STJA0039791)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Phospho-mTOR Ser2481 antibody western blot C6 lysates STJA0036224

Anti-Phospho-mTOR-Ser2481 antibody [R08-9E3] (STJA0036224)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Western blot analysis of various cells using Phospho-mTOR (T2446) Polyclonal Antibody diluted at 1:1000 STJ90544

Anti-Phospho-MTOR-Thr2446 antibody (2412-2461 aa) (STJ90544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-MUC1-Ser1227 antibody (1196-1245 aa) (STJ91261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Immunofluorescence analysis of HepG2 cells, using CD227/MUC1 (Phospho-Tyr1229) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MUC1-Tyr1229 antibody (1201-1250 aa) (STJ91106)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CD227/Mucin 1 (Phospho-Tyr1243) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MUC1-Tyr1243 antibody (1204-1253 aa) (STJ91141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from A431 cells using Phospho-c-Myc-S373 Rabbit pAb (STJ22301). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MYC-S373 antibody (STJ22301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€151.80 - €742.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Myc-S62 Rabbit pAb (STJ22299) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MYC-S62 antibody (STJ22299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of various lysates using Phospho-c-Myc-S62 Rabbit mAb (STJ11102580) at 1:1000 dilution. 293T cells were treated by Forskolin (10 uM) at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-MYC-S62 antibody [S0MR] (STJ11102580)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€142.20 - €625.80

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Ser373) Antibody.

Anti-Phospho-MYC-Ser373 antibody (340-389 aa) (STJ90227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-c-Myc (S62) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MYC-Ser62 antibody (31-80 aa) (STJ91021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Phospho-c-Myc S62 antibody western blot HeLa lysates STJA0035954

Anti-Phospho-Myc-Ser62 antibody [R09-1E2] (STJA0035954)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Phospho-c-Myc Ser62 antibody western blot C6 lysates STJA0034184

Anti-Phospho-Myc-Ser62 antibody [R09-3K1] (STJA0034184)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Mouse/Rat

€160.20 - €337.80

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Western blot analysis of lysates from HT-29 cells, using Phospho-c-Myc-T358 Rabbit pAb (STJ22303). HT-29 cells treated by UV. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MYC-T358 antibody (STJ22303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€151.80 - €742.20

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Western blot analysis of lysates from 293T cells, using Phospho-c-Myc-T58 Rabbit pAb (STJ22298) at 1:500 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-MYC-T58 antibody (STJ22298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of lysates from HeLa cells, using Phospho-c-Myc-T58 Rabbit mAb (STJ11102581) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-MYC-T58 antibody [S1MR] (STJ11102581)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€172.20 - €742.20

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Western blot analysis of lysates from C6 cells, using Phospho-c-Myc-T58/S62 Rabbit mAb (STJ11102579) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-MYC-T58/S62 antibody [S9MR] (STJ11102579)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

€142.20 - €625.80

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Thr358) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MYC-Thr358 antibody (325-374 aa) (STJ90228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-c-Myc (T58) Polyclonal Antibody diluted at 1：500

Anti-Phospho-MYC-Thr58 antibody (25-74 aa) (STJ90229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Phospho-c-Myc antibody western blot HeLa lysates STJA0036004

Anti-Phospho-Myc-Thr58 antibody [R09-1M8] (STJA0036004)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human

€160.20 - €337.80

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Western Blot analysis of 1 A431 cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MYD88-Tyr257 antibody (STJA0009895)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from 293T cells, using Phospho-MYH9-S1943 Rabbit pAb (STJ117900) at 1:2000 dilution. 293T cell lysates were treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-MYH9-S1943 antibody (STJ117900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Phospho-MYH9-S1943 antibody (STJA0005473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€441.00

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Anti-Phospho-MYH9-Ser-1803 antibody (STJA0005471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat

€441.00

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Anti-Phospho-MYH9-Ser-1916 antibody (STJA0005472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat

€441.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MYH9-Ser1943 antibody (STJ196359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Anti-Phospho-MYH9-Ser1943 antibody (STJ196360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot A431 cells using Myosin IIA Heavy Chain phospho-specific antibody STJA0041273

Anti-Phospho-Myosin IIA Heavy Chain, phospho-specific-S1943 antibody (STJA0041273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€651.00

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Western blot Myosin IIA Heavy Chain phospho-specific antibody detection in A431 cells STJA0041283

Anti-Phospho-Myosin IIA Heavy Chain, phospho-specific-Ser-1803 antibody (STJA0041283)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat

€651.00

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Western blot Myosin IIA Heavy Chain phospho-specific antibody detection in A431 cells STJA0041284

Anti-Phospho-Myosin IIA Heavy Chain, phospho-specific-Ser-1916 antibody (STJA0041284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat

€651.00

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Western blot N-WASP phosphorylation states in rat brain lysate with AP treatment STJA0041453

Anti-Phospho-N-WASP-Ser-484/Ser-485 antibody (STJA0041453)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

€651.00

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Western blot WB analysis using antibody N-WASP detection in A431 cells STJA0041455

Anti-Phospho-N-WASP-Tyr-256 antibody (STJA0041455)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

€651.00

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-NACA-Ser43 antibody (9-58 aa) (STJ91263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-NACA-Thr159 antibody (STJA0010000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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phospho-NAK/TBK1 antibody western blot HeLa STJA0035677

Anti-Phospho-NAK/TBK1-Ser172 antibody [R92-5Y-2] (STJA0035677)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Western blot analysis of lysates from HUVEC cells treated with Forskolin 40nM 30', using Nibrin (Phospho-Ser278) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-NBN-Ser278 antibody (251-300 aa) (STJ90727)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from Jurkat cells, using p95/NBS1 (Phospho-Ser343) Antibody. The lane on the left is blocked with the phospho peptide.

Anti-Phospho-NBN-Ser343 antibody (310-359 aa) (STJ90354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€58.80 - €313.20

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Anti-Phospho-NBN-Ser343 antibody (STJ196375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Immunohistochemistry analysis of Phospho-Nucleolin-T76 in paraffin-embedded human lung cancer using Phospho-Nucleolin-T76 Rabbit monoclonal antibody (STJ11105810) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-NCL-T76 antibody [S5810RM] (STJ11105810)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

€142.20 - €625.80

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Western blot analysis of various lysates using Phospho-NEDD4L-S448 Rabbit pAb (STJ11100984) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-NEDD4L-S448 antibody (STJ11100984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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