• Western blot analysis of lysates from ovary cancer, using Myc (Phospho-Thr58) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 293 cells using Phospho-c-Myc (T58) Polyclonal Antibody diluted at 1:500
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Thr58) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-c-Myc (T58) Polyclonal Antibody diluted at 1:500

Anti-Phospho-MYC-Thr58 antibody (25-74 aa) (STJ90229)

SKU:
STJ90229

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Myc proto-oncogene protein-Thr58 (25-74 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IP 2-5 ug mg/lysate
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYC
Gene ID: 4609
Uniprot ID: MYC_HUMAN
Immunogen Region: 25-74 aa
Specificity: Phospho-c-Myc (T58) Polyclonal Antibody detects endogenous levels of c-Myc protein only when phosphorylated at T58.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Myc around the phosphorylation site of Thr58 at the amino acid range 25-74
Function Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CACGATG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis. Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells. Functions with TAF6L to activate target gene expression through RNA polymerase II pause release. Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform.
Protein Name Myc Proto-Oncogene Protein
Class E Basic Helix-Loop-Helix Protein 39
Bhlhe39
Proto-Oncogene C-Myc
Transcription Factor P64
Database Links Reactome: R-HSA-1362277
Reactome: R-HSA-201556
Reactome: R-HSA-2122947
Reactome: R-HSA-2173796
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-4411364
Reactome: R-HSA-5687128
Reactome: R-HSA-5689880
Reactome: R-HSA-6785807
Reactome: R-HSA-69202
Reactome: R-HSA-69656
Reactome: R-HSA-8866911
Reactome: R-HSA-8951430
Reactome: R-HSA-9018519
Reactome: R-HSA-9616222
Reactome: R-HSA-9818749
Cellular Localisation Nucleus
Nucleoplasm
Nucleolus
Alternative Antibody Names Anti-Myc Proto-Oncogene Protein antibody
Anti-Class E Basic Helix-Loop-Helix Protein 39 antibody
Anti-Bhlhe39 antibody
Anti-Proto-Oncogene C-Myc antibody
Anti-Transcription Factor P64 antibody
Anti-MYC antibody
Anti-BHLHE39 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance