Developmental Biology | Research Area | St John's Laboratory

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K5 antibody (STJ11103295) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Acetyl-Histone H4-K5 antibody (STJ11103295)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (STJ11101677) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Acetyl-Histone H4-K5 antibody [S7MR] (STJ11101677)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£118.50 - £521.50

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Anti-Acetyl-Histone H4-K5/K8/K12/K16 antibody [SRM5253] (STJ11107155)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Anti-Acetyl-Histone H4-K56 antibody [SRM14587] (STJ11109317)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA/ChIP/ChIP-seq/CUT&Tag

Reactivity :

Human/Mouse/Rat/Other

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Chromatin immunoprecipitation was performed with 5 Mu g of cross-linked chromatin from HeLa, using 5 Mu g of Acetyl-Histone H4-K8 Rabbit polyclonal antibody (STJ29400) and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

Anti-Acetyl-Histone H4-K8 antibody (STJ29400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Anti-Acetyl-Histone H4-K8 antibody [SRM14937] (STJ11109667)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

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Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K91 Rabbit polyclonal antibody (STJ11100937) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.

Anti-Acetyl-Histone H4-K91 antibody (STJ11100937)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys12) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys12 antibody (10-59 aa) (STJ90128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-tubulin monoclonal antibody (8F11) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys16 antibody (1-50 aa) (STJ90129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys5) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Bcl-2 monoclonal antibody (6B5) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys5 antibody (1-50 aa) (STJ90126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys8) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 monoclonal antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys8 antibody (1-50 aa) (STJ90127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of mouse-lung mouse-kidney mouse-liver lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000

Anti-Acetyl-HNF4A-Lys106 antibody (106 aa) (STJ98691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of K562 cells using Acetyl-HSP 90 (K292/284) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-HSP90AA1-Lys292/284 antibody (251-300 aa) (STJ90144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of A549 cells using Acetyl-HSP 90 (K435) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-HSP90AA1-Lys435 antibody (STJ90145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of PC12 cells using Acetyl-AP-1 (K271) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-JUN-Lys271 antibody (STJ97229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HepG2 cells using Acetyl-PCAF (K428) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-KAT2B-Lys428 antibody (STJ97360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of A549 cells using Acetyl-Cytokeratin 8 (K483) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-KRT8-Lys483 antibody (434-483 aa) (STJ90146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Acetyl-MEF2A-Lys403 antibody (STJA0005976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Acetyl-MYB-Lys471 antibody (STJA0005978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Acetyl-MYB-Lys480 antibody (STJA0005979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Acetyl-MYC-Lys148 antibody (STJA0005980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Acetyl-MYOD1-Lys99/K102 antibody (STJA0005981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Acetyl-NCOA3-Lys616 antibody (STJA0005992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Acetyl-p53-Lys382 antibody [PT0896R] (STJA0039825)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human

£150.00 - £540.00

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Acetyl-RELA-K314/315 antibody (STJ98905)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Acetyl-RELA-K314/315 antibody (STJ98906)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HepG2-UV, mouse brain cells using Acetyl-NF Kappa B-p65 (K122) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-RELA-Lys122 antibody (STJ97231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of Jurkat, K562 cells using Acetyl-NF Kappa B-p65 (K218) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-RELA-Lys218 antibody (181-230 aa) (STJ90149)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HepG2-UV, mouse brain cells using Acetyl-NF Kappa B-p65 (K221) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-RELA-Lys221 antibody (STJ97232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, NF Kappa B-p65 (Acetyl Lys310) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-RELA-Lys310 antibody (275-324 aa) (STJ90133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of HeLa, HepG2 cells using Acetyl-NF Kappa B-p65 (K314/K315) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-RELA-Lys314/Lys315 antibody (STJ97358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Acetyl-RUNX1-Lys24 antibody (STJA0005919)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Acetyl-SRY-Lys136 antibody (STJA0005993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Acetyl-STAT3-Lys49 antibody (STJ193228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Acetyl-STAT3-Lys685 antibody (STJA0005996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of mouse spleen using Acetyl-Stat3 (K87) antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-Acetyl-STAT3-Lys87 antibody (STJ97699)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of MOUSE-HEARTmouse brainusing Acetyl-TAL1/2 (K221/K222/K36/K37) antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-Acetyl-TAL1-Lys37 antibody (STJ97700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1, mouse-liver 2, hela 3, mouse-brain cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-Acetyl-TP53-K120 antibody (STJ98844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of mouse-brain cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-Acetyl-TP53-K305 antibody (STJ98845)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells, using p53 (Acetyl-Lys317) Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Acetyl-TP53-K319 antibody (283-332 aa) (STJ90130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of C6 cells, using Acetyl-p53-K370 antibody (STJ11101783) at 1:1000 dilution. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Acetyl-TP53-K370 antibody [S3MR] (STJ11101783)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Dot-blot analysis of all sorts of peptides using Acetyl-p53-K382 antibody (STJ11103901) at 1:1000 dilution.

Anti-Acetyl-TP53-K382 antibody (STJ11103901)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Acetyl-TP53-K382 antibody (STJ98912)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells, using p53 (Acetyl-Lys386) Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Acetyl-TP53-K386 antibody (344-393 aa) (STJ90132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Acetyl-TP53-Lys164 antibody (STJA0005986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of SKW3 cells using Acetyl-p53 (K320) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-TP53-Lys320 antibody (STJ97359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of Jurkat cells using Acetyl-p53 (K370) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-TP53-Lys370 antibody (331-380 aa) (STJ90150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of Jurkat cells using Acetyl-p53 (K372) Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-TP53-Lys372 antibody (331-380 aa) (STJ98864)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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