• Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 antibody (STJ118031) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse spinal cord using Acetyl-Histone H4-K12 antibody (STJ118031) at dilution of 1:200 (40x lens).
  • Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K12 antibody (STJ118031) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H4-K12 antibody (STJ118031) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H4-K12 antibody (STJ118031) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded human appendix using Acetyl-Histone H4-K12 antibody (STJ118031) at dilution of 1:200 (40x lens).
  • Western blot analysis of extracts of C6 cells, using Acetyl-Histone H4-K12 antibody (STJ118031) at 1:1000 dilution.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
  • Western blot analysis of extracts of C2C12 cells, using Acetyl-Histone H4-K12 antibody (STJ118031) at 1:1000 dilution.C2C12 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.

Anti-Acetyl-Histone H4-K12 antibody (STJ118031)

SKU:
STJ118031
Current Stock:
Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat, Other
Short Description: Rabbit polyclonal primary antibody against Histone H4.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen: A synthetic acetylated peptide around K12 of human Histone H4 (NP_001029249.1).
1 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
6 months standard