Autophagy | Research Area | St John's Laboratory

Western blot analysis of lysates from Hep G2 cells using mTOR Rabbit polyclonal antibody (STJ11105440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MTOR antibody (2400-2500) (STJ11105440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using mTOR antibody (STJ113771) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-mTOR antibody (2400-2500) (STJ113771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of various cells using mTOR Polyclonal Antibody

Anti-MTOR antibody (2447-2496) (STJ94278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Cow/Chicken/Pig

$64.80 - $340.20

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Anti-mTOR antibody [PT0351R] (STJA0039470)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from rat pancreas, using MVB12A Rabbit polyclonal antibody (STJ118378) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-MVB12A antibody (1-273) (STJ118378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$151.88 - $708.08

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Anti-MVB12A antibody (11-61) (STJA0006476)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from SKOV3 cells, using NBR1 Rabbit pAb (STJ11100940) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 90s.

Anti-NBR1 antibody (1-280) (STJ11100940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of PC-12 cells using NBR1 Rabbit polyclonal antibody (STJ24692) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NBR1 antibody (1-966) (STJ24692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-NBR1 antibody (850-900 aa) (STJ13101505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

$447.53

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-NBR1 antibody (STJ197345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of rat-spleen tissue. 1, NBR1 Mouse monoclonal antibody (7C3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-NBR1 antibody (STJ98902)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-OPTN antibody (STJA0010265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-OPTN antibody (STJA0010303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Pan-Alpha Tubulin antibody [SM5020] (STJA0005020)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$315.23

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Western blot analysis of extracts of various cell lines, using DJ-1/PARK7 antibody (STJ11101634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PARK7 antibody (1-100) [S4MR] (STJ11101634)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunofluorescence analysis of L929 cells using DJ-1/PARK7 Rabbit polyclonal antibody (STJ11100527) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PARK7 antibody (1-189) (STJ11100527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of HeLa cells using DJ-1/PARK7 antibody (STJ24895). Blue: DAPI for nuclear staining.

Anti-PARK7 antibody (1-189) (STJ24895)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot analysis of various cells using PARK7 Polyclonal Antibody

Anti-PARK7 antibody (21-70 aa) (STJ94957)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using DJ-1 Polyclonal Antibody

Anti-PARK7 antibody (61-110 aa) (STJ92719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-PARK7 antibody (C-Term) (STJA0005775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$614.93

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Anti-PARK7 antibody [PT1140R] (STJA0039989)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Anti-Parkin antibody (C-Term) (STJ13100219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$447.53

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Anti-Parkin antibody (C-Term) (STJ13100220)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$447.53

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Immunofluorescence analysis of U-2 OS cells using PCNT Rabbit polyclonal antibody (STJ11103192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PCNT antibody (95-212) (STJ11103192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-PCNT antibody (STJ193082)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using PEX5 antibody (STJ114367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PEX5 antibody (364-631) (STJ114367)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of A549 cells using Peroxin 5 Polyclonal Antibody

Anti-PEX5 antibody (540-620 aa) (STJ95042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from C2C12 cells, using Phospho-AMPK Beta 1-S182 Rabbit pAb (STJ11103734) at 1:1000 dilution. C2C12 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-AMPK Beta 1-S182 antibody (STJ11103734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from Mouse heart using Phospho-ATG13-Ser355 Rabbit pAb (STJ11101125) at 1:500 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-ATG13-Ser355 antibody (STJ11101125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-ATG13-Ser355 antibody (STJ196246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-Phospho-ATG14-Ser29 antibody (STJ196247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using (STJ22028) at 1:1000 dilution. 293T cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours. 293T cells were treated by UV at room temperature for 15-30 minutes. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ATM-S1981 antibody (STJ22028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Confocal imaging of paraffin-embedded Human colon tissue (untreated) and Human colon tissue (treated with Lambda pp) using Phospho-ATM-S1981 Rabbit mAb (STJ11102610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-Phospho-ATM-S1981 antibody [S0MR] (STJ11102610)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$197.78 - $867.38

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Anti-Phospho-ATM-Ser-1981 antibody [M366] (STJA0005274)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Mouse/Rat

$493.43

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Anti-Phospho-ATM-Ser-794 antibody (STJA0005273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/FC

Reactivity :

Human/Mouse/Rat

$493.43

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Western Blot analysis of 1 K562 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATM-Ser179 antibody (STJA0010053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of K562 using p-Atm (S1981) antibody. Antibody was diluted at 1:500

Anti-Phospho-ATM-Ser1981 antibody (STJ97691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-ATM-Ser1981 antibody [PT0902R] (STJA0039795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Western Blot analysis of mouse heart, Hela, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATM-Ser367 antibody (STJA0010035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using Phospho-Beclin 1-Ser15 Rabbit pAb (STJ11103769) at 1:500 dilution. NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-Beclin 1-S15 antibody (STJ11103769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from C6 cells using Phospho-Beclin 1-Ser90 Rabbit pAb (STJ11103771) at 1:400 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-Beclin 1-S90 antibody (STJ11103771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-Beclin 1-S90 antibody [SRM] (STJ11107736)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of lysates from HeLa cells, using Phospho-Beclin 1-Ser93 Rabbit pAb (STJ11103786) at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-Beclin 1-S93 antibody (STJ11103786)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-Beclin 1-S93 antibody (STJ11107676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-BECN1-Ser15 antibody (STJ196252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser234. This immunolabeling is decreased in the beclin-1 double alanine substitution mutant (S234A, S295A: Mut) expressed in HeLa cell lysate.

Anti-Phospho-BECN1-Ser234 antibody (STJA0003586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Non-Human Primates/Sheep

$613.58

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Anti-Phospho-BECN1-Ser93 antibody (STJ196253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western Blot analysis of mouse spleen, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BECN1-Ser93/96 antibody (STJA0010075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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