• Western blot analysis of Rat testis, using IFT88 rabbit polyclonal antibody (STJ115021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of 293T, using IFT88 rabbit polyclonal antibody (STJ115021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of L929 cells using IFT88 antibody (STJ115021) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IFT88 antibody (534-833) (STJ115021)

SKU:
STJ115021

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IFT88 (534-833) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IFT88
Gene ID: 8100
Uniprot ID: IFT88_HUMAN
Immunogen Region: 534-833
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 534-833 of human IFT88 (NP_783195.2).
Immunogen Sequence: GLTYEKLNRLDEALDCFLKL HAILRNSAEVLYQIANIYEL MENPSQAIEWLMQVVSVIPT DPQVLSKLGELYDREGDKSQ AFQYYYESYRYFPCNIEVIE WLGAYYIDTQFWEKAIQYFE RASLIQPTQVKWQLMVASCF RRSGNYQKALDTYKDTHRKF PENVECLRFLVRLCTDLGLK DAQEYARKLKRLEKMKEIRE QRIKSGRDGSGGSRGKREGS ASGDSGQNYSASSKGERLS
Tissue Specificity Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.
Function Positively regulates primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.
Protein Name Intraflagellar Transport Protein 88 Homolog
Recessive Polycystic Kidney Disease Protein Tg737 Homolog
Tetratricopeptide Repeat Protein 10
Tpr Repeat Protein 10
Database Links Reactome: R-HSA-5610787
Reactome: R-HSA-5620924
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Reactome: R-HSA-9646399
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Cell Projection
Cilium
Cilium Basal Body
Flagellum
Colocalizes With Entr1 And Gamma-Tubulin At The Basal Body Of Primary Cilia
Colocalizes With Entr1 And Pericentrin At The Centrosome
Alternative Antibody Names Anti-Intraflagellar Transport Protein 88 Homolog antibody
Anti-Recessive Polycystic Kidney Disease Protein Tg737 Homolog antibody
Anti-Tetratricopeptide Repeat Protein 10 antibody
Anti-Tpr Repeat Protein 10 antibody
Anti-IFT88 antibody
Anti-TG737 antibody
Anti-TTC10 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance