Phospho Antibodies | St John's Laboratory

Western blot analysis of various lysates using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (STJ11102558) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60S.

Anti-Phospho-RPS6KB1-T421/S424 antibody [S8MR] (STJ11102558)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunofluorescence analysis of Hela cell. 1, p70 S6 kinase Alpha (phospho Thr229) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-RPS6KB1-Thr229 antibody (195-244 aa) (STJ90375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Pig

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Phospho-RPS6KB1-Thr389 antibody (STJ196372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Cow

$64.80 - $340.20

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Immunofluorescence analysis of Hela cell. 1, p70 S6 kinase Alpha (phospho Thr412) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-RPS6KB1-Thr412 antibody (355-450 aa) (STJ91045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-RPS6KB1-Thr421 antibody (STJ90376)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of LOVO using p-p70 S6 kinase Alpha (T444) antibody. Antibody was diluted at 1:500

Anti-Phospho-RPS6KB1-Thr444 antibody (411-460 aa) (STJ90377)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 5', using p70 S6 Kinase beta (Phospho-Ser423) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6KB2-Ser423 antibody (389-438 aa) (STJ90588)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-Phospho-Rptor-S791 antibody (STJ11107673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot analysis of lysates from 293T cells, using Phospho-Raptor-Ser792 Rabbit pAb (STJ11101062) at 1:1000 dilution. 293T cells were treated by Oligomycin (0. 5 uM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1S.

Anti-Phospho-RPTOR-Ser792 antibody (STJ11101062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RPTOR-Ser792 antibody (STJ196397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western Blot analysis of MCF-7 cell, HepG2 cell, mouse heart tissue, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-RPTOR-Ser863 antibody (STJA0009908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western Blot analysis of A549 cell, 2, LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-RRAS-Tyr66 antibody (STJA0010007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TIF-IA (Phospho-Ser649) Antibody

Anti-Phospho-RRN3-Ser649 antibody (602-651 aa) (STJ90994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-Rsk-1/2/3/4-Ser221/227/S218/232 antibody (191-240 aa) (STJ90596)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of Jurkat cells using Phospho-RUNX1 (S249) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-RUNX1-Ser249 antibody (242-291 aa) (STJ90453)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from Jurkat cells, using AML1 (Phospho-Ser303) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RUNX1-Ser276 antibody (269-318 aa) (STJ90782)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using AML1 (Phospho-Ser435) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RUNX1-Ser435 antibody (401-450 aa) (STJ90619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-RYR2-S2808 antibody (STJ11107697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-RYR2-S2814 antibody (STJ11107660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-RYR2-S2830 antibody (STJ11107661)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Immunohistochemistry analysis of paraffin-embedded human brain, using RyR2 (Phospho-Ser2808) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RYR2-Ser2808 antibody (2774-2823 aa) (STJ91127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western Blot analysis of 1 A549 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-S100A9-Thr113 antibody (STJA0009916)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of HEPG2-UVmouse brainusing p-EDG-1 (T236) antibody. Antibody was diluted at 1:500

Anti-Phospho-S1PR1-Thr236 antibody (206-255 aa) (STJ91353)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-S6 Ribosomal Protein (RPS6)-S235/236-S235/236 antibody [SRM15117] (STJ11109847)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-S6 Ribosomal Protein (RPS6)-S235/236-S235/236 antibody [SRM15118] (STJ11109848)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-S6 Ribosomal Protein (RPS6)-S240/244-S240/244 antibody [SRM15119] (STJ11109849)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 Rabbit mAb (STJ11107738) at 1:54000 dilution incubated overnight at 4C. HeLa, NIH/3T3 and PC-12 cells were treated by 10% FBS at 37C for 30 minutes after serum-starvation overnight. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 30s.

Anti-Phospho-S6 Ribosomal Protein-S240/244 antibody [SRM5547] (STJ11107738)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot of Drosophila S2 cell lysate showing specific labeling of the ~70 kDa p70 S6K protein phosphorylated at Thr398 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+) , but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-S6K-Ser398 antibody (STJA0003773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

D.melanogaster

$613.58

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Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53 kDa S6K1 phosphorylated at Thr449 in the first lane (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide in the third lane (NP).

Anti-Phospho-S6K1-Thr449 antibody (STJA0003774)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Arabidopsis

$613.58

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Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SAMHD1-Thr592 antibody (STJ196411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SATB1-Ser47 antibody (STJ196412)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-SCNN1B-S633 antibody (STJ13102019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC

Reactivity :

Rat/Mouse

$447.53

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Anti-Phospho-SCNN1B-S633 antibody (STJ13102069)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC

Reactivity :

Rat/Mouse

$447.53

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Anti-Phospho-SCNN1B-Thr615 antibody (581-630 aa) (STJ91101)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-SCRIB-Ser1220 antibody (STJ196413)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Syndecan4 (Phospho-Ser179) Antibody

Anti-Phospho-SDC4-Ser179 antibody (145-194 aa) (STJ91310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-SEMA4B-Ser825 antibody (STJ196414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using Phospho-SERCA2/ATP2A2-Thr484 Rabbit pAb (STJ11107681) at 1:1000 dilution. NIH/3T3 cells were treated by ATP (5 mM) at 30 °C for 1 hour. C6 cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-SERCA2/ATP2A2-Thr484 antibody (STJ11107681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of 1) Hela, 2) Rat Brain Tissue, 3) Mouse Brain Tissue with Phosphoserine Mouse mAb diluted at 1:2, 000.

Anti-Phospho-Serine antibody [5B12] (STJ97457)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Species independent

$64.80 - $340.20

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Anti-Phospho-Serine-2-OH antibody [N259/48] (STJA0021097)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

$461.03 - $1,954.13

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Anti-Phospho-Serine-2-OH antibody [N259/52] (STJA0021098)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

$461.03 - $1,954.13

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Anti-Phospho-Serine-2-OMe antibody [N262/16] (STJA0020868)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

$461.03 - $1,954.13

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Anti-Phospho-Serine-2-OMe antibody [N262/20] (STJA0020869)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

$461.03 - $1,954.13

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Anti-Phospho-Serine/Threonine antibody [PT1457R] (STJA0040289)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Western blot analysis of SH-SY5Y 293T using p-Splicing factor 1 (S82) antibody. Antibody was diluted at 1:500

Anti-Phospho-SF1-Ser82 antibody (48-97 aa) (STJ91054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

$64.80 - $340.20

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Western blot analysis of various lysates using Phospho-SF3B1-T313 Rabbit pAb (STJ11100985) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-SF3B1-T313 antibody (STJ11100985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-SF3B1-Thr313 antibody (STJ196415)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western Blot analysis of 1 A431 cell 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SFN-Ser186 antibody (STJA0009865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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