• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-RB-S780 antibody (STJ22373) at dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Immunohistochemistry analysis of Phospho-RB-S780 in paraffin-embedded mouse brain using Phospho-RB-S780 Rabbit polyclonal antibody (STJ22373) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using Phospho-RB-S780 rabbit polyclonal antibody (STJ22373) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-RB-S780 in paraffin-embedded human colon using Phospho-RB-S780 Rabbit polyclonal antibody (STJ22373) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon using Phospho-RB-S780 rabbit polyclonal antibody (STJ22373) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-RB-S780 in paraffin-embedded rat brain using Phospho-RB-S780 Rabbit polyclonal antibody (STJ22373) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using Phospho-RB-S780 rabbit polyclonal antibody (STJ22373) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-RB-S780 Rabbit polyclonal antibody (STJ22373) at dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.

Anti-Phospho-RB1-S780 antibody (STJ22373)

SKU:
STJ22373

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-RB1-S780 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RB1
Gene ID: 5925
Uniprot ID: RB_HUMAN
Immunogen: A synthetic phosphorylated peptide around S780 of human RB (NP_000312.2).
Immunogen Sequence: TLSPI
Tissue Specificity Expressed in the retina. Expressed in foreskin keratinocytes (at protein level).
Post Translational Modifications Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis. N-terminus is methylated by METTL11A/NTM1. Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1. Acetylated during keratinocyte differentiation. Acetylation at Lys-873 and Lys-874 regulates subcellular localization. Can be deacetylated by SIRT1.
Function Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle. The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes. Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription. Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase. RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Protein Name Retinoblastoma-Associated Protein
P105-Rb
P110-Rb1
Prb
Rb
Pp110
Database Links Reactome: R-HSA-113501
Reactome: R-HSA-174178
Reactome: R-HSA-2299718
Reactome: R-HSA-2559584
Reactome: R-HSA-2559585
Reactome: R-HSA-69200
Reactome: R-HSA-69202
Reactome: R-HSA-69231
Reactome: R-HSA-69656
Reactome: R-HSA-8940973
Reactome: R-HSA-9661069
Reactome: R-HSA-9661070
Reactome: R-HSA-9682706
Reactome: R-HSA-9687136
Reactome: R-HSA-9694686
Cellular Localisation Nucleus
During Keratinocyte Differentiation
Acetylation By Kat2b/Pcaf Is Required For Nuclear Localization
Alternative Antibody Names Anti-Retinoblastoma-Associated Protein antibody
Anti-P105-Rb antibody
Anti-P110-Rb1 antibody
Anti-Prb antibody
Anti-Rb antibody
Anti-Pp110 antibody
Anti-RB1 antibody

Information sourced from Uniprot.org

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