Phospho Antibodies | St John's Laboratory

Western blot analysis of LIMK2 (Phospho-Ser283) Antibody. The lane on the right is blocked with the LIMK2 (Phospho-Ser283) peptide.

Anti-Phospho-LIMK2-Ser283 antibody (249-298 aa) (STJ91188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of extracts from HeLa cells, using Phospho-LIMK2-T505 antibody (STJ22229). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-LIMK2-T505 antibody (STJ22229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of various cells using Phospho-LIMK-2 (T505) Polyclonal Antibody

Anti-Phospho-LIMK2-Thr505 antibody (STJ90322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-LIN28A-Ser200 antibody (STJ194717)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using Phospho-HSL-S563 antibody (STJ11100992) at 1:1000 dilution. 3T3L1 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. H9C2 cells were treated with Isoproterenol (10 Mu M for 20 min) at 37 °C. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-LIPE-S563 antibody (STJ11100992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from 3T3-L1 cells, using Phospho-HSL-S660 Rabbit polyclonal antibody (STJ11105787) at 1:600 dilution. 3T3-L1 cells were treated with Isoproterenol (10 µM for 20 min) at 37 °C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-LIPE-S660 antibody (STJ11105787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot analysis of lysates from HeLa cells, using Phospho-HSL-S660 Rabbit polyclonal antibody (STJ11105803) at 1:1000 dilution. HeLa cells were treated by TNF-Alpha (20 ng/ml) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-Phospho-LIPE-S660 antibody (STJ11105803)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of 3T3-L1 cells, using Phospho-HSL-S660 Rabbit monoclonal antibody (STJ11105885) at 1:1000 dilution. 3T3-L1 cells that were not differentiated or differentiated into adipocytes treated with 0. 06mol/L alcohol and treated with isoproterenol (10 Mu M, 20 min). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-LIPE-S660 antibody [S5885RM] (STJ11105885)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$197.78 - $867.38

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-HSL-S853 rabbit monoclonal antibody (STJ11102644) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-Phospho-LIPE-S853 antibody [S4MR] (STJ11102644)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$197.78 - $867.38

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Western blot analysis of 293 cells using Phospho-HSL (S552) Polyclonal Antibody diluted at 1：500

Anti-Phospho-LIPE-Ser552 antibody (518-567 aa) (STJ90796)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of L929 using p-HSL (S855) antibody. Antibody was diluted at 1:1000

Anti-Phospho-LIPE-Ser855 antibody (520-569 aa) (STJ90762)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using Phospho-Lamin A/C-S22 antibody (STJ116397) at 1:2000 dilution or Lamin A antibody (A0249). HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours or treated by Paclitaxel (100 nM/mL) at 37 °C for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours or treated by Paclitaxel (100 nM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 1s.

Anti-Phospho-LMNA-S22 antibody (STJ116397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Rat

$174.83 - $867.38

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Western blot analysis of lysates from HeLa cells treated with paclitaxel 1uM 24h, using Lamin A (Phospho-Ser22) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-LMNA-Ser22 antibody (2-51 aa) (STJ90645)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Lamin A/C (Phospho-Ser392) (PTR1133) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: Hela Lane 3: HEK293Predicted band size: 63, 74kDaObserved band size: 63, 74kDa

Anti-Phospho-LMNA-Ser392 antibody (350-450) [PTR1133] (STJA0006224)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Lamin A/C (Phospho-Ser392) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-LMNA-Ser392 antibody (361-410 aa) (STJ91042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of HeLa cells, using Phospho-LRP6-S1490 antibody (STJ11103731) at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-LRP6-S1490 antibody (STJ11103731)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-LRP6-Ser1490 antibody (STJ196348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-LRP6-Thr1479 antibody (STJA0009851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-LRRK2-S935 rabbit monoclonal antibody (STJ11102647) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnightSecondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-Phospho-LRRK2-S935 antibody [S7MR] (STJ11102647)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$197.78 - $867.38

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Anti-Phospho-LTK-Tyr672/Tyr1278 antibody (STJA0009986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-LYN-Tyr507 antibody (STJ196350)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Lyn (Y508) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-LYN-Tyr508 antibody (463-512 aa) (STJ90764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Lyn-Y396 rabbit monoclonal antibody (STJ11102620) at 1:1000 dilution. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-LYN-Y396 antibody [S0MR] (STJ11102620)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-Phospho-Lyn-Y507 antibody (STJ11107669)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MAD1 (Phospho-Ser428) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MAD1L1-Ser428 antibody (394-443 aa) (STJ91167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using MAK (Phospho-Tyr159) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAK-Tyr159 antibody (126-175 aa) (STJ90583)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-MAP2-Ser136 antibody (STJ196351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

$64.80 - $340.20

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Anti-Phospho-MAP2-Thr1616 antibody (STJ193213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of C6 cells, using Phospho-MEK1-pS221 antibody (STJ22232) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-MAP2K1-pS221 antibody (STJ22232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of HeLa cells, using Phospho-MAP2K1-S217 polyclonal antibody (STJ22230) at 1:1000 dilution or MAP2K1 antibody (A12687). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-MAP2K1-S217 antibody (STJ22230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (STJ22231) at 1:1000 dilution or MAP2K1 antibody (A12687). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight or treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-MAP2K1-S298 antibody (STJ22231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of various cell lines, using Phospho-MEK1-S298 rabbit monoclonal antibody (STJ11102602) at 1:1000 dilution. Both HeLa cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PMA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-Phospho-MAP2K1-S298 antibody [S2MR] (STJ11102602)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$197.78 - $867.38

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Western blot analysis of HEPG2-UV using p-MEK-1 (S298) antibody. Antibody was diluted at 1:500

Anti-Phospho-MAP2K1-Ser298 antibody (264-313 aa) (STJ90489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-T286 antibody (STJ22233) at 1:2000 dilution or MAP2K1 antibody (A12687). HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by ATP (5 mM) at 30 °C for 1 hour. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by ATP (5 mM) at 30 °C for 1 hour. PC-12 cells were treated by Nocodazole (1 Mu g/mL) at 37 °C for 16 hours or treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-MAP2K1-T286 antibody (STJ22233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of C6 cells, using Phospho-MEK1-T292 rabbit monoclonal antibody (STJ11102603) at 1:1000 dilution. C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-Phospho-MAP2K1-T292 antibody [S3MR] (STJ11102603)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of lysates from K562 cells, using MEK1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAP2K1-Thr286 antibody (252-301 aa) (STJ90854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 293 cells using Phospho-MEK-1 (T292) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MAP2K1-Thr292 antibody (261-310 aa) (STJ90326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot of recombinant wild type MEK 1 (WT) and mutant MEK 1 (T292A) (Mut). Specific immunolabeling of the ~45 kDa MEK-1 protein phosphorylated at Thr292 is shown in the second lane where MAP kinase was coexpressed.

Anti-Phospho-MAP2K1-Thr292 antibody (STJA0003684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Bovine/Canine/Non-Human Primates/Rat

$613.58

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Western blot analysis of 3T3-EGF cells using Phospho-MEK-1 (T386) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP2K1-Thr386 antibody (344-393 aa) (STJ90585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot of human T47D cells showing specific immunolabeling of the ~45 kDa MEK 1 protein phosphorylated at Thr386 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-MAP2K1-Thr386 antibody (STJA0003685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Bovine/Canine/Chicken/Non-Human Primates/Mouse/Xenopus

$613.58

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Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (-) or incubated in the presence of serum (+). Immunolabeling of an additional band at ~95 kDa was also observed.

Anti-Phospho-MAP2K1/2-Ser218/222 antibody (STJA0003683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Human/Bovine/Canine/Chicken/Non-Human Primates/Rat/Xenopus/Zebrafish

$613.58

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Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S217/MAP2K2-S221 antibody (STJ22236) at dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST.

Anti-Phospho-MAP2K1/MAP2K2-S217/S221 antibody (STJ22236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts from HepG2 cells using Phospho-MEK2-T394 antibody (STJ22237). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-MAP2K2-T394 antibody (STJ22237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of various cells using Phospho-MEK-2 (T394) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP2K2-Thr394 antibody (261-310 aa) (STJ90329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-MEK-3 (S218) Polyclonal Antibody

Anti-Phospho-MAP2K3-Ser218 antibody (173-222 aa) (STJ90330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MAP2K3-Thr222 antibody (188-237 aa) (STJ90949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-MAP2K3-S189 + MAP2K6-S207 antibody (STJ11101146) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-MAP2K3/MAP2K6-S189/S207 antibody (STJ11101146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-MAP2K3-S189/MAP2K6-S207 antibody (STJ22238) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-MAP2K3/MAP2K6-S189/S207 antibody (STJ22238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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