Phospho Antibodies | St John's Laboratory

Immunofluorescence analysis of HUVEC cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-IL7R-Tyr449 antibody (410-459 aa) (STJ90721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human lymph node, using IL-9R (Phospho-Ser519) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-IL9R-Ser519 antibody (472-521 aa) (STJ91097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using ILK (Phospho-Ser246) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ILK-Ser246 antibody (212-261 aa) (STJ91204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western Blot analysis of 1 HEK-293 cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ILK-Ser343 antibody (STJA0009919)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-INCENP-Thr59 antibody (STJA0009973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-SHIP-1 (Y1021) Polyclonal Antibody diluted at 1：500

Anti-Phospho-INPP5D-Tyr1021 antibody (987-1036 aa) (STJ90494)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from Raji cells, using Phospho-SHIP1-Y1020 Rabbit pAb (STJ116398) at 1:1000 dilution. Raji cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-INPP5D-Y1020 antibody (STJ116398)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-INPPL1-Tyr1135 antibody (STJ196419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA/IHC

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-INPPL1-Tyr986/987 antibody (STJ196420)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot of HeLa cell lysate showing specific labeling of the ~95 kDa IR protein phosphorylated at Thr1160 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-INSR-Thr1160 antibody (STJA0003672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Canine/Non-Human Primates/Mouse/Rat/Xenopus

€568.13

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Western blot analysis of lysates from HepG2 cells, using IR (Phospho-Thr1375) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-INSR-Thr1375 antibody (1331-1380 aa) (STJ90582)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-INSR-Tyr1345 antibody (STJ196330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of 293 cells using Phospho-Insulin R (Y1355) Polyclonal Antibody

Anti-Phospho-INSR-Tyr1355 antibody (1326-1375 aa) (STJ90815)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of Insulin R (phospho Tyr1361) Polyclonal Antibody, using hela, 293 cell treated or untreated with EGF 200ng/ml 30', 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-INSR-Tyr1361 antibody (1331-1380 aa) (STJ90816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-INSR-Tyr999 antibody (STJA0009816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-INSR-Y1146 + Y1131 antibody (STJ11107608)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Anti-Phospho-INSR-Y1151 + Y1136 antibody (STJ11107609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Anti-Phospho-INSR-Y1361 antibody (STJ22190)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Anti-Phospho-Insulin Receptor-Y1345 antibody (STJ11107610)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Anti-Phospho-Integrin Beta 2-Ser745 antibody [PT1301R] (STJA0040103)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemistry analysis of paraffin-embedded human brain, using IRAK1 (Phospho-Ser376) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-IRAK1-Ser376 antibody (342-391 aa) (STJ91205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from Jurkat cells treated with heat shock, using IRAK1 (Phospho-Thr100) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-IRAK1-Thr100 antibody (66-115 aa) (STJ90913)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from HeLa cells, using IRAK1 (Phospho-Thr209) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-IRAK1-Thr209 antibody (175-224 aa) (STJ90531)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRAK1 (Phospho-Thr387) Antibody

Anti-Phospho-IRAK1-Thr387 antibody (353-402 aa) (STJ91323)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from Karpas 299 cells using Phospho-IRAK4-Ser346 Rabbit monoclonal antibody (STJ11105898) at 1:1000 dilution. Karpas 299 cells were treated by IL-1 Beta (200 ng/ml) at 37 °C for 15 minutes after serum starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 75s.

Anti-Phospho-IRAK4-Ser346 antibody [S5898RM] (STJ11105898)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of lysates from Karpas 299 cells using Phospho-IRAK4-Thr345/Ser346 Rabbit polyclonal antibody (STJ11105836) at 1:800 dilution. Karpas 299 cells were treated by IL-1 Beta (10 ng/ml) at 37 °C for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-IRAK4-Thr345/Ser346 antibody (STJ11105836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Anti-Phospho-IRE1 Alpha-Ser724 antibody [PT1195R] (STJA0040223)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-IRE1-Ser724 antibody (STJA0004899)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Human

€568.13

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Western blot analysis of lysates from 293F cells using Phospho-IRF3-S386 Rabbit pAb (STJ11100998) at 1:1000 dilution incubated overnight at 4 °C. 293F cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 5s.

Anti-Phospho-IRF3-S386 antibody (STJ11100998)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from HeLa cells, using Phospho-IRF3-S386 Rabbit mAb (STJ11102584) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-IRF3-S386 antibody [S4MR] (STJ11102584)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-IRF3-S396 Rabbit pAb (STJ113505) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-IRF3-S396 antibody (STJ113505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Immunofluorescence analysis of Hela cell. 1, IRF-3 (phospho Ser385) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). COX IV monoclonal antibody (6C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-IRF3-Ser385 antibody (351-400 aa) (STJ91040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using IRF-3 (Phospho-Ser386) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-IRF3-Ser386 antibody (352-401 aa) (STJ90598)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-Phospho-IRF3-Ser386 antibody [PT0984R] (STJA0039769)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human

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Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using IRF-3 (Phospho-Ser396) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-IRF3-Ser396 antibody (362-411 aa) (STJ90486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-IRF3-Ser396 antibody [PT0875R] (STJA0039777)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

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Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-IRF5-Ser437 antibody (STJA0009884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using Phospho-IRF7-S471/472 Rabbit pAb (STJ11103778) at 1:500 dilution. 293T and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-IRF7-S471/472 antibody (STJ11103778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€161.88 - €803.13

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-IRF7-Ser471/472 antibody (STJ196332)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-Phospho-IRF7-Ser477 antibody (STJ196333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-Phospho-IRF8-S151 antibody (STJ11107724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of lysates from C2C12 cells, using Phospho-IRS1-S1101 Rabbit pAb (STJ11105789) at 1:1000 dilution. C2C12 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-IRS1-S1101 antibody (STJ11105789)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€161.88 - €803.13

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Anti-Phospho-IRS1-S302 antibody (STJ11107637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of various lysates using Phospho-IRS1-S307 (Human Specific) Rabbit pAb (STJ110879) at 1:1000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-IRS1-S307 antibody (STJ110879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Anti-Phospho-IRS1-S307 antibody (STJ11107630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from 293 cells, using Phospho-IRS1-S312 Rabbit pAb (STJ22196). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-IRS1-S312 antibody (STJ22196)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from C2C12 cells using Phospho-IRS1-S612 Rabbit pAb (STJ11102560) at 1:1000 dilution. C2C12 cells were treated by Insulin (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-IRS1-S612 antibody (STJ11102560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from 3T3 cells, using Phospho-IRS1-S636 Rabbit pAb (STJ22197). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-IRS1-S636 antibody (STJ22197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€161.88 - €803.13

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