Anti-Phospho-Histone H1.4-Thr17 antibody [R07-3C1] (STJA0035939)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Phospho-Histone H1.4 (T17)
General Information
| Short Description | Rabbit monoclonal anti-Phospho-Histone H1.4-Thr17 for use in WB, IHC-P, ICC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R07-3C1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | H1-4 |
| Gene ID | 3008 |
| Uniprot ID | H14_HUMAN |
| Immunogen | A synthesized peptide derived from human Phospho-Histone H1.4 (T17) |
Additional Info
| Post Translational Modifications | H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter. Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1. Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. ADP-ribosylated on Ser-150 in response to DNA damage. |
| Function | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. |
| Protein Name | Histone H1.4Histone H1bHistone H1s-4 |
| Database Links | Reactome: R-HSA-140342Reactome: R-HSA-2559584 |
| Cellular Localisation | NucleusChromosomeMainly Localizes In HeterochromatinDysplays A Punctuate Staining Pattern In The Nucleus |
| Alternative Antibody Names | Anti-Histone H1.4 antibodyAnti-Histone H1b antibodyAnti-Histone H1s-4 antibodyAnti-H1-4 antibodyAnti-H1F4 antibodyAnti-HIST1H1E antibody |
Information sourced from Uniprot.org