• Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T3 polyclonal antibody (STJ11100987) at 1:1000 dilution. C6 and HeLa cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. C6 and NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using Phospho-Histone H3-T3 polyclonal antibody (STJ11100987) at 1:1000 dilution or Histone H3 antibody (A2348). Both 293T cells and Raw264. 7 cells were treated by Nocodazole (100 ng/mL) at 37 °C for 16 hours. C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T3 polyclonal antibody (STJ11100987) at 1:1000 dilution or Histone H3 antibody (A2348). Both 293T cells and Raw264. 7 cells were treated by Nocodazole (100 ng/mL) at 37 °C for 16 hours. C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using Phospho-Histone H3-T3 polyclonal antibody (STJ11100987) at 1:1000 dilution. C6 and HeLa cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. C6 and NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-Histone H3-T3 antibody (STJ11100987)

SKU:
STJ11100987

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Histone H3-T3 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around T3 of human HIST1H3A (NP_003520.1).
Immunogen Sequence: MARTK
Background This gene encodes a nuclear protein involved in transcriptional activation. The encoded protein may form a component of several different histone acetyltransferase complexes. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in multiple transcript variants.

Information sourced from Uniprot.org

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