Phospho Antibodies | St John's Laboratory

Anti-Phospho-DYRK2-Tyr382/264 antibody (STJA0009950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells treated with Etoposide 25uM 24h, using E2F1 (Phospho-Thr433) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-E2F1-Thr433 antibody (388-437 aa) (STJ90458)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~50 kDa eEF1A2 protein phosphorylated at Ser358 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase ( Lambda-Ptase, 800 units/1mg protein for 30 min).

Anti-Phospho-EEF1A2-Ser358 antibody (STJA0003624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Chicken/Non-Human Primates/Rat/Sheep/Xenopus

£454.50

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Western blot analysis of extracts of various cell lines, using Phospho-eEF2-T56 antibody (STJ11100973) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight. C6 cells were treated by Forskolin (10uM) for 1 hour after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-EEF2-T56 antibody (STJ11100973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of SKOV3 cells using Phospho-EF-2 (T56) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-EEF2-Thr56 antibody (31-80 aa) (STJ91030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-EEF2-Thr56 antibody (STJ99592)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 antibody (STJ11100986) at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-Phospho-EEF2K-S366 antibody (STJ11100986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-EEF2K-Ser-359 antibody (STJA0005391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-EEF2K-Ser-398 antibody (STJA0005392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-EEF2K-Ser-445 antibody (STJA0005393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Xenopus

£365.50

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Anti-Phospho-EEF2K-Ser-500 antibody (STJA0005394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-EEF2K-Ser-78 antibody (STJA0005389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-EEF2K-Ser359 antibody (336-385 aa) (STJ91253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-eEF2K (S366) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-EEF2K-Ser366 antibody (331-380 aa) (STJ90759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Anti-Phospho-EEF2K-Thr-348 antibody (STJA0005390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of mouse cells using Phospho-Ephrin-B1 (Y317) Polyclonal Antibody

Anti-Phospho-EFNB1-Tyr317 antibody (283-332 aa) (STJ90516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts from HUVEC cells, using phospho-EGFR-S1070 antibody (STJ22101). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-EGFR-S1070 antibody (STJ22101)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-EGFR-Ser-1142 antibody (STJA0005396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-EGFR-Ser-967 antibody (STJA0005397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from HeLa cells treated with TSA 400nM 24H, using EGFR (Phospho-Ser1026) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-EGFR-Ser1026 antibody (991-1040 aa) (STJ90567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-EGFR-Ser1036/1037 antibody (STJ196289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Ser1070) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-EGFR-Ser1070 antibody (1041-1090 aa) (STJ90246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HepG2 cells treated with serum 20% 15', using EGFR (Phospho-Ser1071) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-EGFR-Ser1071 antibody (1036-1085 aa) (STJ90568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293-EGF cells using Phospho-EGFR (S695) Polyclonal Antibody

Anti-Phospho-EGFR-Ser695 antibody (661-710 aa) (STJ90566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of A-431 and HUVEC cells, using Phospho-EGFR-T669 antibody (STJ22097) at 1:1000 dilution. A431 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. HUVEC cells were treated by EGF. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-EGFR-T669 antibody (STJ22097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-EGFR-T678 antibody (STJ11101148) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-EGFR-T678 antibody (STJ11101148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-Phospho-EGFR-T678 antibody (STJ11107618)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-EGFR-Thr669 antibody (STJ196290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-EGFR (T678) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-EGFR-Thr678 antibody (651-700 aa) (STJ90686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-EGFR (T693) Polyclonal Antibody

Anti-Phospho-EGFR-Thr693 antibody (661-710 aa) (STJ90247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-EGFR-Tyr-1101 antibody [M199] (STJA0005398)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot analysis of HELA cells using Phospho-EGFR (Y1016) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-EGFR-Tyr1016 antibody (986-1035 aa) (STJ91037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-EGFR-Tyr1045 antibody (STJ196291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£48.00 - £252.00

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Anti-Phospho-EGFR-Tyr1068 antibody (STJ196292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-EGFR (Y1069) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-EGFR-Tyr1069 antibody (1041-1090 aa) (STJ90515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-EGFR-Tyr1086 antibody (STJ196293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-EGFR (Y1092) Polyclonal Antibody diluted at 1：500

Anti-Phospho-EGFR-Tyr1092 antibody (1061-1110 aa) (STJ90248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-EGFR (Y1110) Polyclonal Antibody diluted at 1：500

Anti-Phospho-EGFR-Tyr1110 antibody (1077-1126 aa) (STJ90249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-EGFR-Tyr1148 antibody (STJ196294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 453 cells using Phospho-EGFR (Y1172) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-EGFR-Tyr1172 antibody (1139-1188 aa) (STJ90250)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-EGFR (Y1197) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-EGFR-Tyr1197 antibody (1161-1210 aa) (STJ90251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-EGFR-Tyr845 antibody (STJ196295)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 453 cells using Phospho-EGFR (Y869) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-EGFR-Tyr869 antibody (836-885 aa) (STJ90252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-EGFR-Tyr992 antibody (STJ196296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-EGFR-Tyr998 antibody (STJ193217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of A-431 cells, using Phospho-EGFR-Y1045 antibody (STJ117917) at 1:1000 dilution or EGFR antibody (A11351). A-431 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-EGFR-Y1045 antibody (STJ117917)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of HeLa cells, using Phospho-EGFR-Y1068 polyclonal antibody (STJ22109) at 1:1000 dilution or EGFR antibody (A11351). HeLa cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Hela cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-EGFR-Y1068 antibody (STJ22109)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts of A-431 cells, using Phospho-EGFR-Y1068 rabbit monoclonal antibody (STJ11102550) at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-EGFR-Y1068 antibody [S0MR] (STJ11102550)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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