Phospho Antibodies | St John's Laboratory

Western blot analysis of lysates from NIH/3T3 cells using Pan Phospho-Serine/Threonine Rabbit pAb (STJ11101030) at 1:400 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 45s.

Anti-Pan-Phospho-p-S/T antibody (STJ11101030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-PKC (pan) (gamma) -T514 Rabbit pAb (STJ29379) at 1:1000 dilution. RAW 264. 7 cells were treated by PMA (200 nM) for 30 minutes. C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Pan-Phospho-PKC Gamma-T514 antibody (STJ29379)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Pan-Phospho-PKC-Beta II-S660 antibody [SRM13709] (STJ11108439)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Pan-Phospho-Serine antibody [SAA0307] (STJA0019180)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/SPR/WB

Reactivity :

Broad-Spectrum

£378.50 - £1,632.50

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Western blot analysis of various lysates using Phospho- (Ser/Thr) ATM/ATR Substrate pAb (STJ11101067) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Pan-Phospho-Serine/Threonine antibody (STJ11101067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from A-431 cells using pan Phospho-Serine/Threonine Mouse mAb (STJ11101105) at 1:1000 dilution incubated overnight at 4 °C. A-431 cells were treated by Calyculin A (50 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/> Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 60s.

Anti-Pan-Phospho-Serine/Threonine antibody [S5MM] (STJ11101105)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£143.50 - £618.50

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Anti-Pan-Phospho-Serine/Threonine antibody [SAA0308] (STJA0019181)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/SPR/WB

Reactivity :

Broad-Spectrum

£378.50 - £1,632.50

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Western blot analysis of lysates from NIH/3T3 cells using Pan Phospho-T Rabbit polyclonal antibody (STJ11105908) at 1:900 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Pan-Phospho-T antibody (STJ11105908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Other

£126.50 - £618.50

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Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine pAb (STJ11101040) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Pan-Phospho-Tyrosine antibody (STJ11101040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Anti-Pan-Phospho-Tyrosine antibody [4G10] (STJA0018700)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Broad-Spectrum

£378.50 - £1,632.50

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Anti-Pan-Phospho-Tyrosine antibody [PY20] (STJA0019177)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC/IP/WB

Reactivity :

Broad-Spectrum

£378.50 - £1,632.50

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Western blot analysis of various lysates using Pan Phospho-Tyrosine Rabbit mAb (STJ11102650) at 1:1000 dilution. Jurkat cells and NIH/3T3 cells and C6 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Pan-Phospho-Tyrosine antibody [S0MR] (STJ11102650)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat/Other

£143.50 - £618.50

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Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine Mouse mAb (STJ11101091) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Pan-Phospho-Tyrosine antibody [S1MM] (STJ11101091)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£118.50 - £521.50

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Anti-Pan-Phospho-Tyrosine antibody [SAA0305] (STJA0019178)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Broad-Spectrum

£378.50 - £1,632.50

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Anti-Pan-Phospho-Tyrosine antibody [SAA0306] (STJA0019179)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Broad-Spectrum

£378.50 - £1,632.50

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Anti-Pan-Phospho-Tyrosine antibody [SRM15116] (STJ11109846)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat/Other

£0.00

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Western blot analysis of lysates from RAW264.7 cells treated with UV 15', using 14-3-3 beta/zeta (Phospho-Ser184/186) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-14-3-3 Beta/Zeta-Ser184/186 antibody (151-200 aa) (STJ90562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunostaining of salamander retina showing labeling of 14-3-3 protein when phosphorylated at Ser58 (STJA0003567, green 1:500) in Müller glial cells. The blue is staining DNA. Photo courtesy of Alex Vila, University of Texas at Houston.

Anti-Phospho-14-3-3-Ser58 antibody (STJA0003567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Non-Human Primates/Sheep/Xenopus/Zebrafish

£454.50

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Anti-Phospho-4E-BP1 )-Thr37/46 antibody [PT0862R] (STJA0039150)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-4E-BP1-Thr69 antibody (35-84 aa) (STJ90163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-4E-BP1-Thr70 antibody [PT1203R] (STJA0040179)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using ABL1 (Phospho-Thr735) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ABL1-Thr735 antibody (701-750 aa) (STJ90579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Anti-Phospho-ABL1-Thr735 antibody (STJ196256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-ABL1-Tyr-245 antibody (STJA0005239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-ABL1-Tyr-412 antibody (STJA0005240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ABL1-Tyr204 antibody (156-205 aa) (STJ90452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells, using c-Abl (Phospho-Tyr245) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ABL1-Tyr245 antibody (196-245 aa) (STJ90694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-ABL1-Tyr245 antibody [PT1402R] (STJA0040363)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human

£150.00 - £540.00

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Anti-Phospho-ABL1-Tyr412 antibody (STJ196258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ABL1-Tyr89 antibody (STJ196259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from C6 cells, using Phospho-c-Abl-Y245 Rabbit pAb (STJ22472). C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3S.

Anti-Phospho-ABL1-Y245 antibody (STJ22000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from C6 cells, using Phospho-c-Abl-Y412 Rabbit pAb (STJ22472). C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-ABL1-Y412 antibody (STJ11101098)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Phospho-c-Abl-Y412 Rabbit pAb (STJ22003). Microwave antigen retrieval performed with 0. 01M Tris/EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Phospho-ABL1-Y412 antibody (STJ22003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Abl (Phospho-Tyr412) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Abl1/2-Tyr393/439 antibody (361-410 aa) (STJ90779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£49.00 - £261.00

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Immunofluorescence analysis of A549 cells, using Abl (Phospho-Tyr393/412) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Abl1/2-Tyr393/439 antibody (406-455 aa) (STJ90164)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from A-431 cells, using Phospho-ACC1-S79 Rabbit pAb (STJ22006) at 1:700 dilution. A-431 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-Phospho-ACACA-S79 antibody (STJ22006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using Phospho-ACC Alpha (S80) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ACACA-Ser80 antibody (46-95 aa) (STJ90755)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 3T3 using p-ACC Alpha (S80) antibody. Antibody was diluted at 1:1000

Anti-Phospho-ACACA-Ser80 antibody (46-95 aa) (STJ90780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Phospho-ACAP1-Ser554 antibody (520-569 aa) (STJ91177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-ACC1/ACC2-S79 antibody [SRM5539] (STJ11107725)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Anti-Phospho-Acetyl CoA Carboxylase 1-S79-S79 antibody [SRM15134] (STJ11109864)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of NIH/3T3, using Phospho-Acetyl CoA Carboxylase-S79 Rabbit polyclonal antibody (STJ11105871) at 1:1000 dilution. NIH/3T3 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-Acetyl CoA Carboxylase-S79 antibody (STJ11105871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£126.50 - £618.50

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Western blot analysis of NIH/3T3, using Phospho-Acetyl CoA Carboxylase-S79 antibody (STJ11105862) at 1:7000 dilution. NIH/3T3 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-Acetyl CoA Carboxylase-S79 antibody [S5862RM] (STJ11105862)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£143.50 - £618.50

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Anti-Phospho-Acetyl-CoA Carboxylase-Ser79 antibody [PT0983R] (STJA0039832)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Western blot analysis of lysates from C6 cells using Phospho-ACLY-S455 Rabbit pAb (STJ116399) at 1:400 dilution. C6 cells were treated by Insulin (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-ACLY-S455 antibody (STJ116399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-ACLY-S455-S455 antibody [SRM15137] (STJ11109867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from COS7 cells treated with Calyculin 50nM 30', using ATP-Citrate Lyase (Phospho-Ser454) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ACLY-Ser455 antibody (420-469 aa) (STJ90846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of IREB1 (Phospho-Ser138) Antibody. The lane on the right is blocked with the IREB1 (Phospho-Ser138) peptide.

Anti-Phospho-ACO1-Ser138 antibody (106-155 aa) (STJ91163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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