• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ACLY-S455 antibody (STJ116399) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunoprecipitation analysis of 200 Mu g extracts of NIH/3T3 cells, using 3 Mu g Phospho-ACLY-S455 polyclonal antibody (STJ116399). Western blot was performed from the immunoprecipitate using Phospho-ACLY-S455 polyclonal antibody (STJ116399) at a dilution of 1:1000. NIH/3T3 cells were treated by Insulin (100 nM) at 37 °C for 10 minutes after serum-starvation overnight.
  • Immunohistochemistry of paraffin-embedded rat pancreas using Phospho-ACLY-S455 antibody (STJ116399) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-ACLY-S455 in paraffin-embedded mouse pancreas using Phospho-ACLY-S455 Rabbit polyclonal antibody (STJ116399) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using Phospho-ACLY-S455 antibody (STJ116399) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-ACLY-S455 in paraffin-embedded mouse brain using Phospho-ACLY-S455 Rabbit polyclonal antibody (STJ116399) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-ACLY-S455 antibody (STJ116399) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-ACLY-S455 in paraffin-embedded rat pancreas using Phospho-ACLY-S455 Rabbit polyclonal antibody (STJ116399) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells, using 3 ug Phospho-ACLY-S455 polyclonal antibody (STJ116399). Western blot was performed from the immunoprecipitate using Phospho-ACLY-S455 polyclonal antibody (STJ116399) at a dilition of 1:1000. NIH/3T3 cells were treated by Insulin (100 nM) at 37 °C for 10 minutes after serum-starvation overnight.
  • Western blot analysis of lysates from HeLa cells using Phospho-ACLY-S455 Rabbit polyclonal antibody (STJ116399) at 1:400 dilution. HeLa cells were treated by Insulin (50 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from NIH/3T3 cells using Phospho-ACLY-S455 Rabbit polyclonal antibody (STJ116399) at 1:400 dilution. NIH/3T3 cells were treated by Insulin (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from C6 cells using Phospho-ACLY-S455 Rabbit polyclonal antibody (STJ116399) at 1:400 dilution. C6 cells were treated by Insulin (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-ACLY-S455 antibody (STJ116399)

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STJ116399

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-ACLY-S455 is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:100
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACLY
Gene ID: 47
Uniprot ID: ACLY_HUMAN
Immunogen: A synthetic phosphorylated peptide around S455 of human ACLY (NP_001087.2).
Immunogen Sequence: PAPSRTASFSESRAD
Post Translational Modifications Phosphorylated by PKA and GSK3 in a sequential manner.phosphorylation results in activation of its activity. Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455. Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues. ISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2. Ubiquitinated at Lys-540, Lys-546 and Lys-554 by the BCR(KLHL25) E3 ubiquitin ligase complex and UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site. BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells.
Function Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.
Protein Name Atp-Citrate Synthase
Atp-Citrate
Pro-S--Lyase
Acl
Citrate Cleavage Enzyme
Database Links Reactome: R-HSA-163765
Reactome: R-HSA-6798695
Reactome: R-HSA-75105
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Atp-Citrate Synthase antibody
Anti-Atp-Citrate antibody
Anti-Pro-S--Lyase antibody
Anti-Acl antibody
Anti-Citrate Cleavage Enzyme antibody
Anti-ACLY antibody

Information sourced from Uniprot.org

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