Western blot analysis of lysates from NIH/3T3 cells usingย Pan Phospho-T Rabbit polyclonal antibody (STJ11105908) atย 1:900 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 ยฐC for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ย  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:ย 180s.

Anti-Pan-Phospho-T antibody (STJ11105908)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11105908
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General Information

Short DescriptionRabbit polyclonal Pan-Phospho-T antibody for use in WB in human, mouse and other samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB
HostRabbit
ReactivityHuman/Mouse/Other
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
FormulationPBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

SpecificityA synthetic peptide corresponding to a sequence containing phosphorylated T.

Citations

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