Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from Rosette leaves, using BIN2 Rabbit polyclonal antibody (STJ11104219) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-BIN2 antibody (281-380) (STJ11104219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

A.thaliana

£146.50 - £642.50

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Western blot analysis of various lysates using BIN3 Rabbit polyclonal antibody (STJ117673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BIN3 antibody (1-253) (STJ117673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BIN3 antibody (184-234 aa) (STJ195772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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STJ71405 (0. 3µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Bin3 antibody (Internal) (STJ71405)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of recombinant Biotin-Goat-Mouse IgG Protein using Biotin Rabbit polyclonal antibody (STJ11101533) at 1:1000 dilution. NA NA Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. NA NA Lysates/proteins: 100 ng per lane. NA NA Blocking buffer: 3% nonfat dry milk in TBST. NA NA Detection: ECL Basic Kit. NA NA Exposure time: 45s.

Anti-Biotin antibody (STJ11101533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Species independent

£112.50 - £524.50

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Anti-Biotin antibody [Hyb-8] (STJ16100225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB/IF/IHC

£287.50 - £1,324.50

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Anti-Biotin antibody [M33] (STJA0019295)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/SPR/WB

Reactivity :

General

£174.50 - £250.50

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Anti-Biotin antibody [M33] (STJA0021648)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

General

£199.50 - £283.50

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Western blot analysis of various lysates, using Biotin Rabbit monoclonal antibody (STJ11103412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Biotin antibody [S2MR] (STJ11103412)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Other

£146.50 - £642.50

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Western blot analysis of various lysates, using Biotin Rabbit monoclonal antibody (STJ11104796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Biotin antibody [S4796RM] (STJ11104796)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Other

£146.50 - £642.50

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Western blot analysis of various lysates using Biotin Rabbit monoclonal antibody (STJ11104797) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Biotin antibody [S4797RM] (STJ11104797)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Other

£146.50 - £642.50

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Anti-Biotin antibody [SAA0349] (STJA0019296)

Clonality :

Monoclonal

Host :

Mouse

Applications :

SPR/WB

Reactivity :

General

£174.50 - £250.50

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STJ72947 (0. 5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-biotinidase antibody (372-385) (STJ72947)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of extracts of various cell lines, using cIAP1 antibody (STJ11101767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-BIRC2 antibody (1-100) [S7MR] (STJ11101767)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using cIAP1 Rabbit pAb (STJ22800) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-BIRC2 antibody (1-300) (STJ22800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of BALA-3T3 cells using cIAP1 rabbit polyclonal antibody (STJ114903) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-BIRC2 antibody (331-618) (STJ114903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-BIRC2 antibody (STJ193113)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of C6 cells using cIAP2 Rabbit polyclonal antibody (STJ117997) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-BIRC3 antibody (100-604) (STJ117997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ72373 (0. 1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-BIRC3 antibody (109-118) (STJ72373)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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Western blot detection of c-IAP2 in Ramos, COS7, Raji and Daudi cell lysates using c-IAP2 mouse mAb (1:1000 diluted).Predicted band size: 68KDa, Observed band size:72KDa.

Anti-BIRC3 antibody [1F12-E5-G12] (STJ99165)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Monkey

£144.00 - £348.00

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Western blot analysis of extracts of various cell lines, using Survivin antibody (STJ11101752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BIRC5 antibody (1-100) [S2MR] (STJ11101752)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using Survivin Rabbit monoclonal antibody (STJ11102164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-BIRC5 antibody (1-140) [S4MR] (STJ11102164)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of MCF7 cells using Survivin Rabbit polyclonal antibody (STJ11105318) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BIRC5 antibody (1-142) (STJ11105318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded mouse fetal liver using Survivin Rabbit polyclonal antibody (STJ26172) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-BIRC5 antibody (1-142) (STJ26172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Survivin antibody (STJ11104675) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-BIRC5 antibody (1-142) [S4675RM] (STJ11104675)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Survivin Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-BIRC5 antibody (11-60 aa) (STJ95837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Survivin Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-BIRC5 antibody (86-135 aa) (STJ95838)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-BIRC5 antibody (C-Term) (STJA0005747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£455.50

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Anti-BIRC5 antibody (STJ503164)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, Survivin monoclonal antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-BIRC5 antibody [Mix] (STJ97436)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat

£48.00 - £252.00

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Anti-BIRC5 antibody {Biotin} (STJ503165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-BIRC5 antibody {FITC} (STJ503166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of extracts of various cell lines, using BIRC7 antibody (STJ28303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-BIRC7 antibody (1-265) (STJ28303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using ML-IAP Polyclonal Antibody

Anti-BIRC7 antibody (162-211) (STJ94147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from CACO2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BIRC8 antibody (122-172 aa) (STJ193880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Birch Pollen antibody [102H10.05] (STJ16102036)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Pollen

£636.50

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Anti-Birch Pollen antibody [110G10.01] (STJ16102037)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Pollen

£636.50

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Anti-Birch Pollen antibody [202B8.03] (STJ16102038)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Pollen

£636.50

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STJ71287 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Bisphosphate 3 NA-nucleotidase antibody (C-Term) (STJ71287)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat

£312.50

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Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BIVM antibody (450-500 aa) (STJ193925)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-BKbeta2 K+ channel antibody [N53/32] (STJA0021565)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Anti-BKbeta4 K+ channel antibody [L18A/3] (STJA0021148)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Anti-BKbeta4 K+ channel antibody [N42/37] (STJA0021149)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Western blot analysis of FABP7 at dilution of 1:1000

Anti-BLBP antibody [SM5213] (STJA0005213)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Immunofluorescence analysis of MCF-7 cells using BRCC2/BLID Rabbit polyclonal antibody (STJ29414). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BLID antibody (1-108) (STJ29414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-BLK antibody (450-500) (STJ500258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-BLK antibody (450-500) {Biotin} (STJ500259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-BLK antibody (450-500) {FITC} (STJ500260)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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