Western Blot Antibodies | St John's Laboratory

Western blot analysis of various lysates using ARFRP1 Rabbit polyclonal antibody (STJ116523) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARFRP1 antibody (1-201) (STJ116523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARFRP1 antibody (110-160 aa) (STJ195125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ARFRP1 antibody [R08-5C5] (STJA0033803)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Rat

£133.50 - £281.50

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Anti-ARFRP1/ARP antibody [SRM13096] (STJ11107826)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunofluorescence analysis of paraffin-embedded human liver cancer using Arginase 1 (ARG1) Rabbit polyclonal antibody (STJ26182) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARG1 antibody (1-322) (STJ26182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Whole cell lysates of HepG2 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Arginase-1 antibody. The HRP-conjugated anti- mouseIgG antibody was used to detect the antibody. Predicted band size: 35 kDa

Anti-ARG1 antibody (200-322 aa) [ABT-Arg1] (STJ197017)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£180.00 - £261.00

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Western blot analysis of 293 cells using Arginase I Polyclonal Antibody

Anti-ARG1 antibody (61-110 aa) (STJ91676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescent image of mouse retina specifically labeling arginase (146-ARG, 1:500, green) and CD206 (red). The blue nuclear stain is DAPI. The image was kindly provided by Sarah Gough, University of Miami Miller School of Medicine.

Anti-ARG1-ARG1 antibody (STJA0003835)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC

Reactivity :

Guinea Pig/Human/Mouse/Rat

£488.50

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Western blot analysis of extracts of Rat kidney, using Arginase 2 (ARG2) antibody (STJ28438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ARG2 antibody (270-354) (STJ28438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using Arginase II Polyclonal Antibody

Anti-ARG2 antibody (305-354 aa) (STJ91677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Arg3.1 antibody (C-Term) (STJ13100597)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Anti-Arg3.1 antibody (STJ13100002)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-Arg3.1 antibody (STJ13100526)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-Arg3.1 antibody (STJ13100537)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-Arg3.1 antibody (STJ13100565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-Arginase 1 (ARG1) antibody [SRM15100] (STJ11109830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemistry analysis of paraffin-embedded human prostate cancer using Arginase 2 (Arginase 2 (ARG2) ) Rabbit monoclonal antibody (STJ11102965) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Arginase 2 antibody (255-354) [S5MR] (STJ11102965)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Arginase 2 antibody [R06-7F9] (STJA0037396)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Anti-Arginase 2 antibody [R07-1V-5] (STJA0033112)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse/Rat

£214.50 - £308.50

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Anti-Arginase 2/ARG2 antibody (23-354aa) [AT7F8] (STJA0041764)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£219.50 - £332.50

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Anti-Arginase 2/ARG2 antibody (23-354aa) [AT7F8] (STJA0041912)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

£238.50 - £361.50

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STJ70719 (0. 01µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Arginase I antibody (C-Term) (STJ70719)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ71134 (0. 05µg/ml) staining of Mouse Liver (A) and Rat Liver (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Arginase, type 1/arg1 (rat)-Rat antibody (C-Term) (STJ71134)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Mouse/Rat

£312.50

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STJ71133 (0. 01µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Arginase, type 1/ARG1 antibody (C-Term) (STJ71133)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Dog

£312.50

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Anti-Arginase-1 antibody (200-322) [ABT-Arg1] (STJA0039040)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF/ELISA

Reactivity :

Human

£49.00 - £261.00

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Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of paraffin-embedded mouse liver using Arginase-1 Rabbit polyclonal antibody (STJ11105172) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Arginase-1 antibody (STJ11105172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Anti-Arginase-1 antibody [PT0364R] (STJA0039493)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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STJ71128 (0. 3µg/ml) staining of A431 (A) and (1ug/ml) NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Argininosuccinate synthetase 1 antibody (Internal) (STJ71128)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Biotinylated STJ71128 (0. 03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-Argininosuccinate synthetase 1, Biotinylated antibody (Internal) {Biotin} (STJ73211)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Anti-ARGLU1 antibody (STJA0008694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Argonaute 2 antibody (N-Term) [11A9] (STJA0041057)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/WB

Reactivity :

Bovine/Human

£474.50

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Anti-Argonaute 2 antibody (STJA0041077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£474.50

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Anti-Argonaute 2 antibody [R09-8P8] (STJA0036275)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Argonaute-2 antibody [SRM14835] (STJ11109565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using RHG1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ARHGAP1 antibody (189-238 aa) (STJ91678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g ARHGAP1 antibody (STJ22672). Western blot was performed from the immunoprecipitate using ARHGAP1 antibody (STJ22672) at a dilution of 1:1000.

Anti-ARHGAP1 antibody (210-439) (STJ22672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ARHGAP10 antibody (STJA0007102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of ARHGAP11A Antibody. The lane on the right is blocked with the ARHGAP11A peptide.

Anti-ARHGAP11A antibody (471-520 aa) (STJ91679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using ARHGAP11B Rabbit polyclonal antibody (STJ119014) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 2s.

Anti-ARHGAP11B antibody (218-267) (STJ119014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-ARHGAP12 antibody (310-390 aa) (STJ91680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ARHGAP15 antibody (STJA0004462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£139.50 - £403.50

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Anti-ARHGAP15 antibody (STJA0007357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from LOVO cells, using RHG17 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ARHGAP17 antibody (331-380 aa) (STJ91681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using ARHGAP18 Polyclonal Antibody

Anti-ARHGAP18 antibody (517-566 aa) (STJ91682)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-ARHGAP20 antibody (800-880 aa) (STJ191317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-ARHGAP21 antibody (STJA0007941)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using ARHGAP22 Rabbit polyclonal antibody (STJ118993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-ARHGAP22 antibody (510-650) (STJ118993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARHGAP22 antibody (565-614 aa) (STJ91683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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