Anti-Arg3.1 antibody (STJ13100002)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from rat Arg3.1 (ARC) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and human.
General Information
| Short Description | Rabbit polyclonal anti-Arg3.1 for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | ARC |
| Gene ID | 23237 |
| Uniprot ID | ARC_HUMAN |
| Immunogen | A synthetic peptide from rat Arg3.1 (ARC) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and human. |
| Specificity | Specific for Arg3.1/ARC. |
Additional Info
| Function | Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory. Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1. In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation. |
| Protein Name | Activity-Regulated Cytoskeleton-Associated ProteinHarcActivity-Regulated Gene 3.1 Protein HomologArc/Arg3.1Arg3.1 |
| Database Links | Reactome: R-HSA-9031628 |
| Cellular Localisation | Extracellular Vesicle MembraneLipid-AnchorPostsynaptic Cell MembraneSynapsePostsynaptic DensityEarly Endosome MembraneCell ProjectionDendriteCytoplasmCytoskeletonCell CortexDendritic SpineCytoplasmic VesicleSecretory VesicleAcrosomeClathrin-Coated Vesicle MembraneForms Virion-Like Extracellular Vesicles That Are Released From NeuronsEnriched In Postsynaptic Density Of Dendritic SpinesTargeted To Inactive Synapses Following Interaction With Camk2b In The Kinase Inactive StateAccumulation At Weaker Synapses May Be Required To Prevent Their Undesired EnhancementAssociated With The Cell Cortex Of Neuronal Soma And DendritesAssociated With The Sperm Tail |
| Alternative Antibody Names | Anti-Activity-Regulated Cytoskeleton-Associated Protein antibodyAnti-Harc antibodyAnti-Activity-Regulated Gene 3.1 Protein Homolog antibodyAnti-Arc/Arg3.1 antibodyAnti-Arg3.1 antibodyAnti-ARC antibodyAnti-KIAA0278 antibody |
Information sourced from Uniprot.org