Anti-ARHGAP10 antibody (STJA0007102)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human ARHGAP10
General Information
| Short Description | Rabbit polyclonal anti-Rho GTPase-activating protein 10 for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARHGAP10 |
| Gene ID | 79658 |
| Uniprot ID | RHG10_HUMAN |
| Immunogen | Synthesized peptide derived from human ARHGAP10 |
| Specificity | This antibody detects endogenous levels of ARHGAP10 at Human, Mouse |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylated in vitro by constitutive active PKN3. |
| Function | GTPase-activating protein that catalyzes the conversion of active GTP-bound Rho GTPases to their inactive GDP-bound form, thus suppressing various Rho GTPase-mediated cellular processes. Also converts Cdc42 to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death. Associates with MICAL1 on the endosomal membrane to promote Rab8-Rab10-dependent tubule extension. After dissociation with MICAL1, recruits WDR44 which connects the endoplasmic reticulum (ER) with the endosomal tubule, thereby participating in the export of a subset of neosynthesized proteins. |
| Protein Name | Rho Gtpase-Activating Protein 10Gtpase Regulator Associated With Focal Adhesion Kinase 2Graf2Graf-Related Protein 2Rho-Type Gtpase-Activating Protein 10 |
| Database Links | Reactome: R-HSA-211728Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149 |
| Cellular Localisation | CytoplasmPerinuclear RegionCell MembraneEndosome MembraneAssociation To Cell Membrane Is Dependent On Ph DomainColocalized With Mical1Rab8aRab8b And Rab10 On Endosomal Tubules |
| Alternative Antibody Names | Anti-Rho Gtpase-Activating Protein 10 antibodyAnti-Gtpase Regulator Associated With Focal Adhesion Kinase 2 antibodyAnti-Graf2 antibodyAnti-Graf-Related Protein 2 antibodyAnti-Rho-Type Gtpase-Activating Protein 10 antibodyAnti-ARHGAP10 antibodyAnti-GRAF2 antibody |
Information sourced from Uniprot.org