Western Blot Antibodies | St John's Laboratory

Anti-ARAF antibody (N-Term) [M289] (STJA0005557)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

$440.91

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Anti-ARAP1 antibody (794-844 aa) (STJ192917)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using ARAP1 antibody (STJ112495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-ARAP1 antibody (834-1133) (STJ112495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

$152.49 - $697.59

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Anti-ARAP2 antibody (STJA0009264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$67.62 - $360.18

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Anti-ARAP3 antibody (C-Term) (STJ70429)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IP

Reactivity :

Human/Mouse/Dog/Pig/Cow

$431.25

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Anti-ARAP3 antibody (STJA0008431)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of various lysates using ARC Rabbit pAb (STJ111600) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ARC antibody (1-220) (STJ111600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARC antibody (70-150 aa) (STJ192386)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-ARC antibody [PT1315R] (STJA0040251)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Immunofluorescence analysis of C6 cells using ARCN1 Rabbit polyclonal antibody (STJ117191) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARCN1 antibody (212-511) (STJ117191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-ARCN1 antibody (STJA0007762)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Ard1 antibody at 1:2500 dilution; 50 ng of protein per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-ARD1 antibody (STJ140150)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Archeoprepona

$186.99 - $544.41

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Anti-AREB6 antibody [PT0216R] (STJA0039659)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

$207.00 - $745.20

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STJ71831 (0. 05µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AREB6/ZEB1 antibody (Internal) (STJ71831)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse/Rat

$431.25

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Western blot analysis of HBE cells using Amphiregulin Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-AREG antibody (91-140 aa) (STJ97339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-AREL1 antibody (354-404 aa) (STJ198007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using ARF1 antibody (STJ11104363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-ARF1 antibody (1-181) (STJ11104363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using ARF1 antibody (STJ22666) at 1:500 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.

Anti-ARF1 antibody (100-181) (STJ22666)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using ARF1 Rabbit mAb (STJ11102451) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-ARF1 antibody (70-150) [S1MR] (STJ11102451)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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STJ71062 (1µg/ml) staining of A431 (A) and (0. 3ug/ml) of HeLa (B) and MCF7 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ARF1 antibody (C-Term) (STJ71062)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/FC

Reactivity :

Human/Mouse/Rat/Dog

$431.25

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Anti-ARF1 antibody (STJ500153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IP/WB

Reactivity :

Human/Mouse/Rat

$643.77

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Anti-ARF1 antibody (STJA0006869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-ARF1 antibody {Biotin} (STJ500154)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IP/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Anti-ARF1 antibody {FITC} (STJ500155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IP/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using ARF12 Rabbit polyclonal antibody (STJ11104022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ARF12 antibody (710-818 aa) (STJ11104022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

O.sativa

$198.03 - $853.53

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Anti-ARF3 antibody (66-116) (STJA0006333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of NIH/3T3 cells using ARF4 Rabbit polyclonal antibody (STJ117836) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARF4 antibody (1-180) (STJ117836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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STJ72838 (0. 5µg/ml) staining of NIH3T3 (A) and HeLa (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ARF4 antibody (137-150) (STJ72838)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Pig/Cow

$431.25

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARF4 antibody (71-120 aa) (STJ91673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-ARF4 antibody (STJ500156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$589.95

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Anti-ARF4 antibody {Biotin} (STJ500157)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Anti-ARF4 antibody {FITC} (STJ500158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Western blot analysis of lysates from wild type (WT) and ARF5 knockout (KO) 293T cells, using [KO Validated] ARF5 Rabbit polyclonal antibody (STJ11100862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ARF5 antibody (1-180) (STJ11100862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of extracts of various cell lines, using ARF5 antibody (STJ22668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ARF5 antibody (1-180) (STJ22668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARF5 antibody (40-120 aa) (STJ190524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Confocal imaging of NIH/3T3 cells using ARF5 Rabbit monoclonal antibody (STJ11101858, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-ARF5 antibody (81-180) [S8MR] (STJ11101858)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunofluorescence analysis of HeLa cells using ARF6 antibody (STJ22670) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ARF6 antibody (1-175) (STJ22670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARF6 antibody (30-110 aa) (STJ91674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of C6 cells using ARF6 Rabbit monoclonal antibody (STJ11101343) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ARF6 antibody (76-175) [S3MR] (STJ11101343)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody (STJ29198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ARFGAP1 antibody (1-220) (STJ29198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARFGAP1 antibody (171-220 aa) (STJ91671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Cow

$67.62 - $360.18

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Immunofluorescence analysis of U-2 OS cells using ARFGAP2 Rabbit polyclonal antibody (STJ119821) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARFGAP2 antibody (242-521) (STJ119821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-ARFGAP2 antibody (STJA0009100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-ARFGAP3 antibody (280-360 aa) (STJ91672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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STJ70078 staining (2µg/ml) of mouse brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-ARFGAP3 antibody (C-Term) (STJ70078)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

$431.25

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Western blot analysis of various lysates using ARFGEF1 Rabbit polyclonal antibody (STJ118910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ARFGEF1 antibody (220-420) (STJ118910)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of various cells using BIG2 Polyclonal Antibody

Anti-ARFGEF2 antibody (1491-1540 aa) (STJ91854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded Human thymus gland. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARFIP1 antibody (271-320 aa) (STJ91675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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