Western Blot Antibodies | St John's Laboratory

Anti-CD8 antibody [4B11] (STJ16100850)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human

£1,892.50

Choose Options

Anti-CD8 beta antibody (70-120) (STJ505974)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

Choose Options

Anti-CD8 beta antibody (70-120) {Biotin} (STJ505975)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Anti-CD8 beta antibody (70-120) {FITC} (STJ505976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Immunohistochemistry analysis of CD80 in paraffin-embedded human spleen using CD80 Rabbit polyclonal antibody (STJ118479) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD80 antibody (1-100) (STJ118479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunohistochemistry of rat spleen with Anti-CD80 primary antibody (STJ500483) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-CD80 antibody (1-100) (STJ500483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Mouse/Rat

£427.50

Choose Options

Anti-CD80 antibody (1-100) {Biotin} (STJ500484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Mouse/Rat

£528.50

Choose Options

Anti-CD80 antibody (1-100) {FITC} (STJ500485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Mouse/Rat

£528.50

Choose Options

Flow cytometry:1X10^6 C2C12 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (2 Mu g/mL, left) or CD80 Rabbit monoclonal antibody (STJ11105206, 2 Mu g/mL, right).

Anti-CD80 antibody (38-245) [S5206RM] (STJ11105206)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

STJ72065 (1µg/ml) staining of Lymph Node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD80 antibody (Internal) (STJ72065)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/FC

Reactivity :

Human

£312.50

Choose Options

Western blot analysis of various lysates, using CD80 Rabbit monoclonal antibody antibody (STJ11105144) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CD80 antibody [S5144RM] (STJ11105144)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

Choose Options

Western blot analysis of various lysates, using CD81 Rabbit monoclonal antibody (STJ11102173) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD81 antibody (100-200) [S3MR] (STJ11102173)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot analysis of Rat brain, using CD81 Rabbit polyclonal antibody (STJ11105352) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CD81 antibody (106-211) (STJ11105352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-CD81 antibody (111-160 aa) (STJ96759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Anti-CD81 antibody (114-199 aa) [SRM] (STJ11106904)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Immunofluorescence analysis of THP-1 and U-937 cells using CD81 Rabbit polyclonal antibody (STJ111247) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD81 antibody (114-199) (STJ111247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-CD81 antibody (15-205 aa) (STJ140295)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Reacts against human, rat, mouse, canine and monkey proteins.

£135.50 - £394.50

Choose Options

Western blot analysis of CD81 in C2C12, 3T3, MCF-7 lysates using CD81 antibody.

Anti-CD81 antibody (34-63+113-201 aa) [R02-3D6] (STJA0013040)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse

£264.50

Choose Options

Anti-CD81 antibody [1.3.3.22] (STJ16100041)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human/Mouse/Rat

£287.50 - £1,324.50

Choose Options

Anti-CD81 antibody [1D6] (STJ16100575)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IP/FC/IF

Reactivity :

Human/Sheep/Chimpanzee/Goat

£741.50

Choose Options

Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD82 antibody (120-200 aa) (STJ190278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded mouse brain using CD82 antibody (STJ28271) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CD82 antibody (150-250) (STJ28271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates, using CD82 Rabbit mAb (STJ11102466) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-CD82 antibody (168-267) [S6MR] (STJ11102466)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

Choose Options

Anti-CD82 antibody (170-220 aa) (STJ13100999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

Choose Options

Anti-CD82 antibody (220-267 aa) (STJ13100998)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

Choose Options

Immunofluorescence analysis of paraffin-embedded mouse spleen using CD83 Rabbit monoclonal antibody (STJ11102023) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD83 antibody (1-100) [S3MR] (STJ11102023)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Western blot analysis of various cells using CD83 Polyclonal Antibody diluted at 1：500

Anti-CD83 antibody (101-150 aa) (STJ92144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of various lysates, using CD83 Rabbit monoclonal antibody (STJ11104824) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CD83 antibody (20-143) [S4824RM] (STJ11104824)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

Choose Options

Western blot analysis of various lysates using CD83 Rabbit pAb (STJ23013) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CD83 antibody (20-205) (STJ23013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

STJ73194 (1µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD83 antibody (97-106) (STJ73194)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Cow

£312.50

Choose Options

Western blot analysis of K562, L929 cells using CD84 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD84 antibody (131-180 aa) (STJ96621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of HeLa cells using CD84 antibody (STJ28516). Blue: DAPI for nuclear staining.

Anti-CD84 antibody (22-225) (STJ28516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Western blot analysis of extracts of various cell lines, using CD84 antibody (STJ11103482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD84 antibody (246-345) [S2MR] (STJ11103482)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Anti-CD84 antibody [153-4D9] (STJ16100008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

£287.50 - £1,324.50

Choose Options

Western blot analysis of extracts of various cell lines, using CD86 antibody (STJ11101564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CD86 antibody (1-100) [S4MR] (STJ11101564)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot analysis of various lysates, using CD86 Rabbit polyclonal antibody (STJ11105173) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD86 antibody (24-245 aa) (STJ11105173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of CD86 in paraffin-embedded mouse spleen using CD86 Rabbit polyclonal antibody (STJ113561) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CD86 antibody (24-247) (STJ113561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of Raji cells using CD86 Rabbit polyclonal antibody (STJ23014) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD86 antibody (25-133) (STJ23014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells using 3 Mu g CD86 antibody (STJ119185). Western blot was performed from the immunoprecipitate using CD86 antibody (STJ119185) at a dilution of 1:1000.

Anti-CD86 antibody (25-329) (STJ119185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot with Anti-CD86 Antibody (STJ500486) with peptide on at 1:500 dilution in buffer.

Anti-CD86 antibody (250-329) (STJ500486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£427.50

Choose Options

Anti-CD86 antibody (250-329) {Biotin} (STJ500487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

Choose Options

Anti-CD86 antibody (250-329) {FITC} (STJ500488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

Choose Options

Anti-CD86 antibody (STJA0010129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis using CD86 monoclonal antibody against L1210 (1) and MOLT-4 (2) cell lysate.

Anti-CD86 antibody [1B3] (STJ97939)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Anti-CD86 antibody [BU63] (STJA0001270)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/Block/IP

Reactivity :

Human

£123.50 - £187.50

Choose Options

Anti-CD88 antibody [P12/1] (STJ16100547)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC/IHC-F

Reactivity :

Human/Rhesus

£938.50

Choose Options

Anti-CD88 antibody [S5/1] (STJ16100838)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC

Reactivity :

Human/Rabbit/Ferret/Bovine/Mustelid/Mink

£938.50

Choose Options

Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD8 a (ABT155) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MOLT-4Predicted band size: 26kDaObserved band size: 35kDa

Anti-CD8A antibody (100-235 aa) [ABT155] (STJ197126)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

Choose Options