Anti-Cardiac Troponin I antibody [4D3-F3] (STJA0033264)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenSynthetic peptides corresponding to the sequence of human Cardiac Troponin I.
General Information
| Short Description | Mouse monoclonal anti-Cardiac Troponin I for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 4D3-F3 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Concentration | 4 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | TNNI3 |
| Gene ID | 7137 |
| Uniprot ID | TNNI3_HUMAN |
| Immunogen | Synthetic peptides corresponding to the sequence of human Cardiac Troponin I. |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-42 and Ser-44 by PRKCE.phosphorylation increases myocardium contractile dysfunction. Phosphorylated at Ser-23 and Ser-24 by PRKD1.phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). |
| Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Protein Name | Troponin I - Cardiac MuscleCardiac Troponin I |
| Database Links | Reactome: R-HSA-390522Reactome: R-HSA-5578775 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Troponin I - Cardiac Muscle antibodyAnti-Cardiac Troponin I antibodyAnti-TNNI3 antibodyAnti-TNNC1 antibody |
Information sourced from Uniprot.org