| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CASC5 (1-300) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KNL1 |
| Gene ID: |
57082 |
| Uniprot ID: |
KNL1_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CASC5 (NP_733468.3). |
| Immunogen Sequence: |
MDGVSSEANEENDNIERPVR RRHSSILKPPRSPLQDLRGG NERVQESNALRNKKNSRRVS FADTIKVFQTESHMKIVRKS EMEGCSAMVPSQLQLLPPGF KRFSCLSLPETETGENLLLI QNKKLEDNYCEITGMNTLLS APIHTQMQQKEFSIIEHTRE RKHANDQTVIFSDENQMDLT SSHTVMITKGLLDNPISEKS TKIDTTSFLANLKLHTEDSR MKKEVNFSVDQNTSSENKI |
| Tissue Specificity | Highly expressed in testis, where it is localized in germ cells, in particular in spermatocytes and in the pre-acrosome of round spermatids. Detected in the acrosome of ejaculated spermatozoa. Detected in adult thymus, bone marrow, colon, small intestine, appendix and placenta, and in fetal liver and thymus. |
| Function | Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore. |
| Protein Name | Kinetochore Scaffold 1All1-Fused Gene From Chromosome 15q14 ProteinAf15q14Bub-Linking Kinetochore ProteinBlinkinCancer Susceptibility Candidate Gene 5 ProteinCancer/Testis Antigen 29Ct29Kinetochore-Null Protein 1Protein Casc5Protein D40/Af15q14 |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-606279Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreWeakly Expressed In Interphase NucleiExpression Increases From Prophase To Late AnaphaseBut Greatly Diminishes From The Telophase And Cytokinesis To Early G1 Phase Of Cell Cycle |
| Alternative Antibody Names | Anti-Kinetochore Scaffold 1 antibodyAnti-All1-Fused Gene From Chromosome 15q14 Protein antibodyAnti-Af15q14 antibodyAnti-Bub-Linking Kinetochore Protein antibodyAnti-Blinkin antibodyAnti-Cancer Susceptibility Candidate Gene 5 Protein antibodyAnti-Cancer/Testis Antigen 29 antibodyAnti-Ct29 antibodyAnti-Kinetochore-Null Protein 1 antibodyAnti-Protein Casc5 antibodyAnti-Protein D40/Af15q14 antibodyAnti-KNL1 antibodyAnti-CASC5 antibodyAnti-KIAA1570 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
