• Immunofluorescence analysis of HeLa cells using CARS antibody (STJ28623). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CARS antibody (STJ28623) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CARS antibody (469-748) (STJ28623)

SKU:
STJ28623

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CARS (469-748) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CARS1
Gene ID: 833
Uniprot ID: SYCC_HUMAN
Immunogen Region: 469-748
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 469-748 of human CARS (NP_001742.1).
Immunogen Sequence: ILRAPVDITGQFEKWGEEEA ELNKNFYDKKTAIHKALCDN VDTRTVMEEMRALVSQCNLY MAARKAVRKRPNQALLENIA LYLTHMLKIFGAVEEDSSLG FPVGGPGTSLSLEATVMPYL QVLSEFREGVRKIAREQKVP EILQLSDALRDNILPELGVR FEDHEGLPTVVKLVDRNTLL KEREEKRRVEEEKRKKKEEA ARRKQEQEAAKLAKMKIPPS EMFLSETDKYSKFDENGLP
Function Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).
Protein Name Cysteine--Trna Ligase - Cytoplasmic
Cysteinyl-Trna Synthetase
Cysrs
Database Links Reactome: R-HSA-379716
Reactome: R-HSA-9725370
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Cysteine--Trna Ligase - Cytoplasmic antibody
Anti-Cysteinyl-Trna Synthetase antibody
Anti-Cysrs antibody
Anti-CARS1 antibody
Anti-CARS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance